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Fig. 2 | BMC Microbiology

Fig. 2

From: A novel high-throughput assay to quantify the vaccine-induced inhibition of Bordetella pertussis adhesion to airway epithelia

Fig. 2

Standard curve showing the relationship between bacteria-associated fluorescence and number of bacteria. Labeling of B. pertussis Tohama I with Alexa Fluor® 488 was performed as described in Materials and Methods. Fluorescent bacteria were suspended in PBS to reach OD600 0.2 and then 2-fold serially diluted. For each dilution, 100 μL of bacterial suspension were transferred in triplicates in a 96-well plate and fluorescence was assessed at 485/535 nm by a multi-well fluorescent reader, while 100 μL were serially diluted and plated on BG agar plates in triplicates for CFU counting. Results represent mean ± SD from one out of the two representative experiments performed in triplicates

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