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Table 1 Monoisotopic calculated and experimental mass analyses from MALDI-MS of two repeat unit (2RU) oligosaccharides obtained from O-antigen polysaccharide enzymatic cleavage with 9NATSP

From: Bacteriophage tailspike protein based assay to monitor phase variable glucosylations in Salmonella O-antigens

Oligosaccharide compositiona [M + Na]+ calc/Da [M + Na]+ exp/Da
Salmonella strain
Heidelberg 586 Brancaster Paratyphi B
2 RU 1241.45 1241.45 1241.44 1241.44
2 RU + 2Glc–Abeb 1435.41 n.d. c 1435.47 1435.44
2 RU + 2Glc 1565.56 n.d. c 1565.53 1565.52
2 RU + 2Glc + Ac 1607.57 n.d. c 1607.53 1607.52
  1. atwo repeat units of basic repeat α-d-Galp-1-2-α-d-Manp(−α-3-1-Abep)-1-4-α-l-Rhap-1-3- as produced by TSP cleavage [44], Abe: Abequose, Ac: Acetylation
  2. bwithout Abequose
  3. cnot detected