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Table 1 Transformation of P. palmivora using various concentrations of A. tumefaciens EHA105 expressing pCB301TOR-GFP

From: Establishment of a simple and efficient Agrobacterium-mediated transformation system for Phytophthora palmivora

OD600 of Agrobacteria Data from 1 representative experiment Data from 3 experiments
Zoospore concentration (/ml) Zoospore volume (ml) Number of G418-resistant transformants Number of transformants with detectable GFP signal Number of G418-resistant transformants/107 zoospores Average number of G418-resistant transformants/107 zoospores
0 3.3 × 106 2.5 0 0 0 0
0.1 3.3 × 106 2.5 13 10 16 22 ± 5
0.2 3.3 × 106 2.5 16 15 19 21 ± 2
0.4 3.3 × 106 2.5 25 23 30 27 ± 5
0.8 3.3 × 106 2.5 13 13 16 18 ± 4