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Table 2 Representative proteins differentially expressed in the WT and sav1322 mutant strains

From: The SAV1322 gene from Staphylococcus aureus: genomic and proteomic approaches to identification and characterization of gene function

Spot ID Accessiona Protein name Protein Score Gene Locusb Expression change
Carbohydrate metabolism and transport
A023 gi|15924402 Dihydrolipoamide succinyltransferase 714 odhB SAV1412 Down
A025 gi|15924086 Dihydrolipoamide dehydrogenase 1153 pdhD SAV1096 Down
A277 gi|15924841 Fumarate hydratase 601 fumC SAV1851 Down
A043 gi|15924684 Isocitrate dehydrogenase 845 citC SAV1694 Down
A286 gi|15924412 Glucose-specific enzyme II, PTS system A component 297 crr SAV1422 Down
A287 gi|15924701 Acetate kinase 750 ackA SAV1711 Up
Energy metabolism
A029 gi|15925597 Malate:quinone oxidoreductase 1353 mqo2 SAV2607 Down
A055 gi|15923231 L-lactate dehydrogenase 298 lctE SAV0241 Down
A056 gi|15923246 Xylitol dehydrogenase 78   SAV0256 Down
A063 gi|15924332 Transketolase 684 tkt SAV1342 Down
A071 gi|15925406 Phosphglyceromutase 364 gpmA SAV2416 Down
A183 gi|15924687 Pyruvate kinase 433 pykA SAV1697 Down
A008 gi|15923216 Formate acetyltrasferase 1155 pflB SAV0226 Up
A058 gi|15925514 D-lactate dehydrogenase 972 Ddh SAV2524 Up
A078 gi|15925115 Fructose-bisphosphate aldolase 62 fbaA SAV2125 Up
A281 gi|15923595 Alcohol dehydrogenase 1305 Adh SAV0605 Up
Lipid metabolism
A027 gi|15924291 Glycerol kinase 1037 glpK SAV1301 Down
A054 gi|15923640 Dihydroxyacetone kinase subunit DhaK 420   SAV0650 Down
A067 gi|15924001 Enoyl-(acyl carrier protein) reductase 526 fabI SAV1011 Down
A072 gi|15923029 Glycerophosphoryl diester phosphodiesterase-like protein 616   SAV0039 Down
A089 gi|15925088 (3R)-hydroxymyristoyl-ACP dehydrogenase 125 fabZ SAV2098 Up
Amino-acid biosynthesis/metabolism
A039 gi|15923948 NAD-specific glutamate dehydrogenase 970 gudB SAV0958 Down
A046 gi|15923947 Ornithine--oxo-acid transaminase 1027 rocD SAV0957 Down
A045 gi|15925103 Serine hydroxymethyltransferase 365 glyA SAV2113 Down
A049 gi|15924192 Carbamoyl phosphate synthase small subunit 923 pyrAA SAV1202 Up
A051 gi|15924159 Ornithine carbamoyltransferase 556 argF SAV1169 Up
A060 gi|15924160 Carbamate kinase 990 arcC1 SAV1170 Up
A280 gi|15924727 Bifunctional 3-deoxy-7-phophoheptulonate sunthase/chorismate mutase 434   SAV1737 Up
Cell division, cell wall, cell envelope biogenesis
A017 gi|15925144 Glucosamine--fructiose-6-phosphate aminotransferase 167 glmS SAV2154 Down
A032 gi|15925072 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase 792 murF SAV2082 Down
A033 gi|15925114 UDP-N-acetylglucosamine-1-carboxylvinyl transferase 1062 murZ SAV2124 Down
A048 gi|15923145 Capsular polysaccharide synthesis enzyme Cap5G 518 capG SAV0155 Down
A274 gi|15924665 Trigger factor 1278 tig SAV1675 Down
Purine and pyrimidine metabolism
A079 gi|15924058 Phosphoribosylformylglycinamidine synthase 162 purQ SAV1068 Down
A035 gi|15924191 Dihydroorotase 866 pyrC SAV1201 Up
A059 gi|15924190 Aspartate carbamoyltranferase 328 pyrB SAV1200 Up
A080 gi|15925102 Uracil phosphoribosyltransferase 206 Upp SAV2112 Up
Transcription and translation
A021 gi|15923537 Elongation factor G 411 fusA SAV0547 Down
A031 gi|15923518 Glutamyl-tRNA synthetase 264 gltX SAV0528 Down
A053 gi|15924247 Elongation factor Ts 317 Tsf SAV1257 Down
A061 gi|15924246 30S ribosomal protein S2 465 rpsB SAV1256 Down
A065 gi|15923491 50S ribosomal protein L25 424 rplY SAV0501 Down
A069 gi|15923538 Elongation factor Tu 123 Tuf SAV0548 Down
A070 gi|15925240 50S ribosomal protein L3 516 rplC SAV2250 Down
A076 gi|15924709 30S ribosomal protein S4 532 rpsD SAV1719 Down
A096 gi|15924253 Prolyl-tRNA synthetase 176 proS SAV1263 Down
A108 gi|15925214 DNA-directed RNA polymerase subunit alpha 905 rpoA SAV2224 Down
A276 gi|15924256 Transcription elongation factor NusA 931 nusA SAV1266 Down
A030 gi|15924889 Aspartyl/glutamyl-tRNA amidotrasferase subunit B 336 gatB SAV1899 Up
A081 gi|15922999 Seryl-tRNA synthetase 379 serS SAV0009 Up
A087 gi|15923529 50S ribosomal protein L10 292 rplJ SAV0539 Up
Stress response and heat shock
A066 gi|15923693 Oxidoreductase 242   SAV0703 Down
A084 gi|15924414 Methionine sulfoxide reductase A 605 msrA2 SAV1424 Down
A090 gi|15924413 Methionine sulfoxide reductase B 342 msrB SAV1423 Down
A138 gi|15925129 General stress protein 20U 519 dps SAV2139 Down
A012 gi|15925538 ATP-dependent Clp proteinase chain 2194 clpL SAV2548 Up
Others
A042 gi|15924396 Tellurite resistance protein 716   SAV1406 Down
A064 gi|15923958 5-oxo-1,2,5-tricarboxilic-3-penten acid decarboxylase 123   SAV0968 Down
A088 gi|15924853 Bacterioferritin comigratory protein 959   SAV1863 Down
A016 gi|15923990 Thimet oligopeptidase-like protein 733   SAV1000 Up
A020 gi|15925280 Urease subunit alpha 862 ureC SAV2290 Up
A044 gi|15923092 Aminoacylase 628   SAV0102 Up
A091 gi|15925281 Urease accessory protein UreE 209 ureE SAV2291 Up
A114 gi|15925318 Dehydrogenase 389   SAV2328 Up
A285 gi|15924697 Metal-dependent hydrolase 609   SAV1707 Up
Unknown function
A040 gi|15924926 Hypothetical protein 700 - SAV1936 Down
A062 gi|15923824 Hypothetical protein SAV0834 537 - SAV0834 Down
A074 gi|15924667 Hypothetical protein SAV1677 436 - SAV1677 Down
A075 gi|15925559 Hypothetical protein SAV2569 203 - SAV2569 Down
A083 gi|15924605 Hypothetical protein SAV1615 91 - SAV1615 Down
A086 gi|15924342 Hypothetical protein SAV1352 256 - SAV1352 Down
A085 gi|15925677 Hypothetical protein SA2687 746 - SAV2687 Up
  1. aAccession numbers are from the NCBI protein database
  2. bDesignate ORF number/gene locus in the S. aureus Mu50 genome