The SAV1322 gene from Staphylococcus aureus : genomic and proteomic approaches to identification and characterization of gene function

Jung Wook Kim; Hyun-Kyung Kim; Gi Su Kang; Il-Hwan Kim; Hwa Su Kim; Yeong Seon Lee; Jae Il Yoo

doi:10.1186/s12866-016-0824-2

Table 2 Representative proteins differentially expressed in the WT and sav1322 mutant strains

From: The SAV1322 gene from Staphylococcus aureus: genomic and proteomic approaches to identification and characterization of gene function

Spot ID	Accession^a	Protein name	Protein Score	Gene	Locus^b	Expression change
Carbohydrate metabolism and transport
A023	gi\|15924402	Dihydrolipoamide succinyltransferase	714	odhB	SAV1412	Down
A025	gi\|15924086	Dihydrolipoamide dehydrogenase	1153	pdhD	SAV1096	Down
A277	gi\|15924841	Fumarate hydratase	601	fumC	SAV1851	Down
A043	gi\|15924684	Isocitrate dehydrogenase	845	citC	SAV1694	Down
A286	gi\|15924412	Glucose-specific enzyme II, PTS system A component	297	crr	SAV1422	Down
A287	gi\|15924701	Acetate kinase	750	ackA	SAV1711	Up
Energy metabolism
A029	gi\|15925597	Malate:quinone oxidoreductase	1353	mqo2	SAV2607	Down
A055	gi\|15923231	L-lactate dehydrogenase	298	lctE	SAV0241	Down
A056	gi\|15923246	Xylitol dehydrogenase	78		SAV0256	Down
A063	gi\|15924332	Transketolase	684	tkt	SAV1342	Down
A071	gi\|15925406	Phosphglyceromutase	364	gpmA	SAV2416	Down
A183	gi\|15924687	Pyruvate kinase	433	pykA	SAV1697	Down
A008	gi\|15923216	Formate acetyltrasferase	1155	pflB	SAV0226	Up
A058	gi\|15925514	D-lactate dehydrogenase	972	Ddh	SAV2524	Up
A078	gi\|15925115	Fructose-bisphosphate aldolase	62	fbaA	SAV2125	Up
A281	gi\|15923595	Alcohol dehydrogenase	1305	Adh	SAV0605	Up
Lipid metabolism
A027	gi\|15924291	Glycerol kinase	1037	glpK	SAV1301	Down
A054	gi\|15923640	Dihydroxyacetone kinase subunit DhaK	420		SAV0650	Down
A067	gi\|15924001	Enoyl-(acyl carrier protein) reductase	526	fabI	SAV1011	Down
A072	gi\|15923029	Glycerophosphoryl diester phosphodiesterase-like protein	616		SAV0039	Down
A089	gi\|15925088	(3R)-hydroxymyristoyl-ACP dehydrogenase	125	fabZ	SAV2098	Up
Amino-acid biosynthesis/metabolism
A039	gi\|15923948	NAD-specific glutamate dehydrogenase	970	gudB	SAV0958	Down
A046	gi\|15923947	Ornithine--oxo-acid transaminase	1027	rocD	SAV0957	Down
A045	gi\|15925103	Serine hydroxymethyltransferase	365	glyA	SAV2113	Down
A049	gi\|15924192	Carbamoyl phosphate synthase small subunit	923	pyrAA	SAV1202	Up
A051	gi\|15924159	Ornithine carbamoyltransferase	556	argF	SAV1169	Up
A060	gi\|15924160	Carbamate kinase	990	arcC1	SAV1170	Up
A280	gi\|15924727	Bifunctional 3-deoxy-7-phophoheptulonate sunthase/chorismate mutase	434		SAV1737	Up
Cell division, cell wall, cell envelope biogenesis
A017	gi\|15925144	Glucosamine--fructiose-6-phosphate aminotransferase	167	glmS	SAV2154	Down
A032	gi\|15925072	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase	792	murF	SAV2082	Down
A033	gi\|15925114	UDP-N-acetylglucosamine-1-carboxylvinyl transferase	1062	murZ	SAV2124	Down
A048	gi\|15923145	Capsular polysaccharide synthesis enzyme Cap5G	518	capG	SAV0155	Down
A274	gi\|15924665	Trigger factor	1278	tig	SAV1675	Down
Purine and pyrimidine metabolism
A079	gi\|15924058	Phosphoribosylformylglycinamidine synthase	162	purQ	SAV1068	Down
A035	gi\|15924191	Dihydroorotase	866	pyrC	SAV1201	Up
A059	gi\|15924190	Aspartate carbamoyltranferase	328	pyrB	SAV1200	Up
A080	gi\|15925102	Uracil phosphoribosyltransferase	206	Upp	SAV2112	Up
Transcription and translation
A021	gi\|15923537	Elongation factor G	411	fusA	SAV0547	Down
A031	gi\|15923518	Glutamyl-tRNA synthetase	264	gltX	SAV0528	Down
A053	gi\|15924247	Elongation factor Ts	317	Tsf	SAV1257	Down
A061	gi\|15924246	30S ribosomal protein S2	465	rpsB	SAV1256	Down
A065	gi\|15923491	50S ribosomal protein L25	424	rplY	SAV0501	Down
A069	gi\|15923538	Elongation factor Tu	123	Tuf	SAV0548	Down
A070	gi\|15925240	50S ribosomal protein L3	516	rplC	SAV2250	Down
A076	gi\|15924709	30S ribosomal protein S4	532	rpsD	SAV1719	Down
A096	gi\|15924253	Prolyl-tRNA synthetase	176	proS	SAV1263	Down
A108	gi\|15925214	DNA-directed RNA polymerase subunit alpha	905	rpoA	SAV2224	Down
A276	gi\|15924256	Transcription elongation factor NusA	931	nusA	SAV1266	Down
A030	gi\|15924889	Aspartyl/glutamyl-tRNA amidotrasferase subunit B	336	gatB	SAV1899	Up
A081	gi\|15922999	Seryl-tRNA synthetase	379	serS	SAV0009	Up
A087	gi\|15923529	50S ribosomal protein L10	292	rplJ	SAV0539	Up
Stress response and heat shock
A066	gi\|15923693	Oxidoreductase	242		SAV0703	Down
A084	gi\|15924414	Methionine sulfoxide reductase A	605	msrA2	SAV1424	Down
A090	gi\|15924413	Methionine sulfoxide reductase B	342	msrB	SAV1423	Down
A138	gi\|15925129	General stress protein 20U	519	dps	SAV2139	Down
A012	gi\|15925538	ATP-dependent Clp proteinase chain	2194	clpL	SAV2548	Up
Others
A042	gi\|15924396	Tellurite resistance protein	716		SAV1406	Down
A064	gi\|15923958	5-oxo-1,2,5-tricarboxilic-3-penten acid decarboxylase	123		SAV0968	Down
A088	gi\|15924853	Bacterioferritin comigratory protein	959		SAV1863	Down
A016	gi\|15923990	Thimet oligopeptidase-like protein	733		SAV1000	Up
A020	gi\|15925280	Urease subunit alpha	862	ureC	SAV2290	Up
A044	gi\|15923092	Aminoacylase	628		SAV0102	Up
A091	gi\|15925281	Urease accessory protein UreE	209	ureE	SAV2291	Up
A114	gi\|15925318	Dehydrogenase	389		SAV2328	Up
A285	gi\|15924697	Metal-dependent hydrolase	609		SAV1707	Up
Unknown function
A040	gi\|15924926	Hypothetical protein	700	-	SAV1936	Down
A062	gi\|15923824	Hypothetical protein SAV0834	537	-	SAV0834	Down
A074	gi\|15924667	Hypothetical protein SAV1677	436	-	SAV1677	Down
A075	gi\|15925559	Hypothetical protein SAV2569	203	-	SAV2569	Down
A083	gi\|15924605	Hypothetical protein SAV1615	91	-	SAV1615	Down
A086	gi\|15924342	Hypothetical protein SAV1352	256	-	SAV1352	Down
A085	gi\|15925677	Hypothetical protein SA2687	746	-	SAV2687	Up

^aAccession numbers are from the NCBI protein database
^bDesignate ORF number/gene locus in the S. aureus Mu50 genome

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