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Table 2 Affinity of ulcer secretions to the immobilised antibodies in SPR system and the correlation to culture results

From: In vitro model of production of antibodies; a new approach to reveal the presence of key bacteria in polymicrobial environments

Ulcer secretion Culture results Binding to the produced antibodies in SPR system
Anti-P. aeruginosa Anti-S. aureus Anti-E. fecalis Anti-S. epidermidis
Ulcer secretion from patients with chronic ulcer
1 Staphylococcus aureus + Enterobacter cloacae negative positive negative positive
2 Staphylococcus aureus + Echerichia coli negative positive negative negative
3 Staphylococcus aureus + Pseudomonas aeroginosa negative positive negative negative
4 Staphylococcus aureus negative negative negative negative
5 Proteus mirabilis + coagulase negative staphylococcus negative negative positive positive
6 negative negative negative negative negative
7 Pseudomonas aeruginosa + Enterococcus fecalis positive negative positive negative
8 Staphylococcus aureus negative positive negative positive
9 Staphylococcus aureus negative positive positive positive
10 negative negative negative negative negative
Ulcer secretion from patients post breast cancer surgery
1 negative negative negative negative positive
2 negative negative negative negative positive
3 negative negative negative negative positive
4 Staphylococcus aureus negative positive negative negative
5 Staphylococcus aureus negative positive negative negative
Ulcer secretion from healthy volunteers post skin biopsy in fore-arm
1 negative negative negative negative positive
2 negative negative negative negative negative
3 negative negative negative negative negative
4 negative negative negative negative positive
5 negative negative negative negative positive
6 negative negative negative negative positive
7 negative negative negative negative positive
8 negative negative negative negative positive
9 negative negative negative negative positive