In vitro model of production of antibodies; a new approach to reveal the presence of key bacteria in polymicrobial environments

Wu, Chongcong; Nakka, Sravya; Mansouri, Sepahdar; Bengtsson, Torbjörn; Nayeri, Tayeb; Nayeri, Fariba

doi:10.1186/s12866-016-0821-5

Table 2 Affinity of ulcer secretions to the immobilised antibodies in SPR system and the correlation to culture results

From: In vitro model of production of antibodies; a new approach to reveal the presence of key bacteria in polymicrobial environments

Ulcer secretion	Culture results	Binding to the produced antibodies in SPR system
Ulcer secretion	Culture results	Anti-P. aeruginosa	Anti-S. aureus	Anti-E. fecalis	Anti-S. epidermidis
Ulcer secretion from patients with chronic ulcer
1	Staphylococcus aureus + Enterobacter cloacae	negative	positive	negative	positive
2	Staphylococcus aureus + Echerichia coli	negative	positive	negative	negative
3	Staphylococcus aureus + Pseudomonas aeroginosa	negative	positive	negative	negative
4	Staphylococcus aureus	negative	negative	negative	negative
5	Proteus mirabilis + coagulase negative staphylococcus	negative	negative	positive	positive
6	negative	negative	negative	negative	negative
7	Pseudomonas aeruginosa + Enterococcus fecalis	positive	negative	positive	negative
8	Staphylococcus aureus	negative	positive	negative	positive
9	Staphylococcus aureus	negative	positive	positive	positive
10	negative	negative	negative	negative	negative
Ulcer secretion from patients post breast cancer surgery
1	negative	negative	negative	negative	positive
2	negative	negative	negative	negative	positive
3	negative	negative	negative	negative	positive
4	Staphylococcus aureus	negative	positive	negative	negative
5	Staphylococcus aureus	negative	positive	negative	negative
Ulcer secretion from healthy volunteers post skin biopsy in fore-arm
1	negative	negative	negative	negative	positive
2	negative	negative	negative	negative	negative
3	negative	negative	negative	negative	negative
4	negative	negative	negative	negative	positive
5	negative	negative	negative	negative	positive
6	negative	negative	negative	negative	positive
7	negative	negative	negative	negative	positive
8	negative	negative	negative	negative	positive
9	negative	negative	negative	negative	positive

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