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Table 2 Recognition of EPIYA-phosphopeptides and phosphorylated CagA proteins by commercial α-phosphotyrosine antibodies

From: Systematic analysis of phosphotyrosine antibodies recognizing single phosphorylated EPIYA-motifs in CagA of East Asian-type Helicobacter pylori strains

Phospho-antibody name Company name Recognition of phosphopeptides Relative phosphorylation signal intensity of translocated CagA by H. pylori strains
   EPIYA-A EPIYA-B EPIYA-D Ind69 F453 Mand38 CH7 TN2-GF4 2002-14 Shi470
α-PY-99 Santa Cruz Biotech +++ +++ +++ +++ +++ +++ +++ +++ +++ +++
α-PY-20 (BD) BD Biosciences +++ +++ +++ +/- ++ +/- +++ +++ +++ +++
α-PY-20 (SC) Santa Cruz Biotech +++ +++ +++ +/- ++ +/- ++ +++ +++ +++
α-PY-100 Cell Signaling +++ + +++ - + - + ++ ++ ++
α-PY-69 BD Biosciences +++ ++ ++ + ++ ++ ++ +++ +++ ++
α-PY-102 Cell Signaling +++ - ++ +/- + +/- + ++ ++ +++
α-PY-350 Santa Cruz Biotech - - - - - - - - - -
  1. Abbreviations: PY (phosphotyrosine), EPIYA motif (glutamic acid-proline-isoleucine-tyrosine-alanine phosphorylation motif in CagA), Antibody recognition: +++ (strong signal); ++ (moderate signal); + (weak signal); - (no signal)