Systematic analysis of phosphotyrosine antibodies recognizing single phosphorylated EPIYA-motifs in CagA of East Asian-type Helicobacter pylori strains

Table 2 Recognition of EPIYA-phosphopeptides and phosphorylated CagA proteins by commercial α-phosphotyrosine antibodies

Phospho-antibody name	Company name	Recognition of phosphopeptides			Relative phosphorylation signal intensity of translocated CagA by H. pylori strains
		EPIYA-A	EPIYA-B	EPIYA-D	Ind69	F453	Mand38	CH7	TN2-GF4	2002-14	Shi470
α-PY-99	Santa Cruz Biotech	+++	+++	+++	+++	+++	+++	+++	+++	+++	+++
α-PY-20 (BD)	BD Biosciences	+++	+++	+++	+/-	++	+/-	+++	+++	+++	+++
α-PY-20 (SC)	Santa Cruz Biotech	+++	+++	+++	+/-	++	+/-	++	+++	+++	+++
α-PY-100	Cell Signaling	+++	+	+++	-	+	-	+	++	++	++
α-PY-69	BD Biosciences	+++	++	++	+	++	++	++	+++	+++	++
α-PY-102	Cell Signaling	+++	-	++	+/-	+	+/-	+	++	++	+++
α-PY-350	Santa Cruz Biotech	-	-	-	-	-	-	-	-	-	-

Abbreviations: PY (phosphotyrosine), EPIYA motif (glutamic acid-proline-isoleucine-tyrosine-alanine phosphorylation motif in CagA), Antibody recognition: +++ (strong signal); ++ (moderate signal); + (weak signal); - (no signal)

ISSN: 1471-2180