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Fig. 6 | BMC Microbiology

Fig. 6

From: Systematic analysis of phosphotyrosine antibodies recognizing single phosphorylated EPIYA-motifs in CagA of East Asian-type Helicobacter pylori strains

Fig. 6

CagA phosphorylation at EPIYA-motifs during H. pylori infection of AGS cells was investigated using seven different α-phosphotyrosine antibodies. Seven different CagA-expressing East Asian-type H. pylori strains as well as the T4SS-inactive negative control (Shi470ΔcagL) were used for infection studies on AGS cells. The infection was monitored over 6 h and the samples shown in Fig. 5 were harvested after photographing. Tyrosine phosphorylation of EPIYA-motifs in CagA of the seven different strains was analyzed with the indicated α-phosphotyrosine antibodies as previously described [48]. Presence of equal amounts of CagA from each sample was approved using a monoclonal α-CagA antibody. The ~120-170 kDa section of the gels is shown. Arrows indicate the phospho-CagA bands, while red asterisks mark bands of various tyrosine-phosphorylated host cell proteins

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BMC Microbiology

ISSN: 1471-2180

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