Fig. 3

Comparison of phospho-signal intensities for East Asian- and Western-type EPIYA motifs by seven commercial α-phosphotyrosine antibodies. a Schematic presentation of CagA EPIYA-motifs, comprising either the EPIYA-A, EPIYA-B and EPIYA-C motif in Western-type H. pylori strains as here shown for the strain 26695 or the East Asian-type CagA EPIYA-motifs in which the EPIYA-C region is replaced by EPIYA-D as found in strain TN2-GF4. b Quantified spot intensities of East Asian-type derived EPIYA-motifs A, B and D were probed with seven commercially available phosphotyrosine antibodies as indicated. The Chemidoc imager was used to measure densitometrically the percentage of phosphorylation of each sample. The data are representative from three independent experiments, where the strongest spot on each Dotblot was set at 100 %. c Quantification of spot intensities of corresponding phosphotyrosine peptides derived from Western-type EPIYA-motifs A, B and C. These data were taken from our previous study [48]