Fig. 4
From: Disruption of the carA gene in Pseudomonas syringae results in reduced fitness and alters motility
Expression of P32 and carA. a Regions of varying lengths upstream to the carA coding region were cloned into lux reporter constructs; the regions they span are shown graphically. The inverted arrows represent the predicted stem-loop of the Rho-independent terminator. (b) Expression from lux promoter fusions were evaluated in VBMM medium (black squares), VBMM supplemented with 40 mM arginine (dark gray triangles), VBMM supplemented with 10 mM uracil (light gray circles), and VBMM supplemented with 40 mM arginine and 10 mM uracil (dark gray diamonds). Data shown are the least squares means (LS Means) with standard error of normalized luminescence (lux) values over time, derived from at least 3 independent biological replicates for each promoter fusion-medium combination, each containing 2–3 technical replicates. Note that the scales for each panel are different in order to clearly show statistically different data points. For each time point, the values which are significantly different from VBMM are shown with an asterisk (using Tukey HSD, α = 0.05). Normalized luminescence (lux) is the ratio of luminescence to OD600. Statistical analysis was performed using the program JMP Pro11