Fig. 3From: Disruption of the carA gene in Pseudomonas syringae results in reduced fitness and alters motilityCo-transcription of P32 and carA. a Map of the genomic region containing dapB, P32 and carA in DC3000. The locations and orientations of RT- and PCR primers are indicated. b Agarose gel electrophoresis result of the RT-PCR experiments using the primers pairs indicated. The expected length of the PCR products for the primer pairs are as follows: Primer pair 1 and 2, ~54Â bps; primer pair 1 and 4, ~370Â bps; and primer pair 3 and 4, ~209Â bps. Control reactions in which reverse transcriptase was omitted were performed for each primer set and RNA sampleBack to article page