Table 2 Ct-values of the three DNA extraction protocols of the MTC-specific target genes Heli and IS 1081 generated with the field tissue samples of cattle
Animal No. | Tissue | Weight in grams | Protocol 1 | Protocol 2 | Protocol 3 | Protocol 1 | Protocol 2 | Protocol 3 | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
Heli | IS 1081 | Heli | IS 1081 | Heli | IS 1081 | Overall result per animal | |||||
1 | Lung | 5 | no ct | no ct | no ct | no ct | – | – | negative | negative | – |
Diaphragm | 5 | no ct | no ct | no ct | no ct | – | – | ||||
2 | Lung | 5 | no ct | no ct | no ct | no ct | – | – | negative | negative | – |
3 | Intestinal ln | 3 | no ct | no ct | no ct | no ct | – | – | negative | negative | negative |
Kidney | 3 | no ct | no ct | no ct | no ct | no ct | no ct | ||||
Kidney ln | 2 | no ct | no ct | no ct | no ct | no ct | no ct | ||||
4 | Intestinal ln | 5 | no ct | no ct | no ct | no ct | – | – | negative | negative | negative |
Liver | 4 | no ct | no ct | no ct | no ct | no ct | no ct | ||||
5 | Ln cervicalis superf. | 8 | no ct | no ct | no ct | no ct | no ct | no ct | negative | negative | negative |
Mediastinal ln | 10 | no ct | no ct | no ct | no ct | no ct | no ct | ||||
Kidney | 7 | no ct | no ct | no ct | no ct | – | – | ||||
Kidney ln | 4 | no ct | no ct | no ct | no ct | – | – | ||||
6 | Liver | 5 | no ct | no ct | no ct | no ct | – | – | negative | negative | negative |
Lung | 2 | no ct | no ct | no ct | no ct | no ct | no ct | ||||
Kidney | 4 | no ct | no ct | no ct | no ct | – | – | ||||
7 | Lunga | 3 | no ct | no ct | no ct | no ct | no ct | no ct | negative | negative | – |
8 | Lung ln | 6 | 27.20 | 26.96 | 31.60 | 32.50 | – | – | positive | positive | – |
9 | Retropharyngeal ln | 3 | 37.99 | 37.58 | 36.93 | 37.64 | – | – | positive | positive | – |
10 | Intestinal ln | 4 | 31.10 | 30.54 | 28.87 | 29.32 | 31.85 | 32.08 | positive | positive | positive |
11 | Liver ln | 4 | 43.92 | 37.32 | 35.59 | 35.00 | 37.54 | 39.29 | positive | positive | positive |
Lung | 2 | 34.26 | 31.18 | 37.77 | 39.37 | 37.56 | 38.68 | ||||
12 | Intestinal ln | 5 | 28.21 | 26.69 | 31.10 | 31.50 | – | – | positive | positive | – |
13 | Intestinal ln | 6 | 29.23 | 28.02 | 38.42 | 39.25 | 35.53 | 35.65 | positive | equivocal | positive |
14 | Intestinal ln | 4 | no ct | no ct | 33.90 | 33.92 | 33.97 | 34.20 | negative | positive | positive |
15 | Diaphragm | 10 | no ct | no ct | no ct | no ct | no ct | no ct | negative | negative | negative |
Diaphragm | 10 | no ct | no ct | no ct | no ct | no ct | no ct | ||||
Diaphragm | 3 | no ct | no ct | no ct | no ct | – | – | ||||
16 | Intestinal ln | 3 | 35.31 | 35.60 | no ct | no ct | 33.41 | 34.74 | positive | positive | positive |
Lung | 3 | no ct | no ct | no ct | no ct | no ct | no ct | ||||
Lung ln | 3 | 29.71 | 30.38 | 30.45 | 31.05 | 30.56 | 30.90 | ||||
Spleen | 6 | no ct | no ct | no ct | no ct | 41.09 | 37.00 | ||||
Kidney | 8 | no ct | no ct | no ct | no ct | no ct | no ct | ||||
17 | Intestinal ln | 3 | 34.31 | 34.36 | 32.00 | 32.20 | – | – | positive | positive | – |
18 | Liver | 4 | no ct | no ct | no ct | no ct | – | – | positive | positive | positive |
Lunga | 5 | 26.53 | 27.10 | 24.80 | 24.86 | 26.28 | 26.73 | ||||
Mediastinal ln | 8 | 30.22 | 30.40 | 32.66 | 33.19 | 32.57 | 32.82 | ||||
Spleen | 5 | no ct | no ct | 34.50 | 35.70 | – | – | ||||
Kidney | 3 | no ct | no ct | 38.80 | 38.20 | – | – | ||||
Retropharyngeal ln | 2 | no ct | no ct | ne | ne | – | – | ||||
19 | Mediastinal ln | 3 | no ct | no ct | no ct | no ct | no ct | no ct | negative | negative | negative |
20 | Intestinal ln | 5 | 32.95 | 32.87 | 33.80 | 34.40 | – | – | positive | positive | – |
Mediastinal ln | 5 | 35.88 | 36.29 | 38.70 | 40.90 | – | – | ||||
Retropharyngeal ln | 4 | no ct | no ct | no ct | no ct | – | – | ||||
21 | Lung ln | 4 | no ct | no ct | 29.29 | 30.08 | 31.59 | 31.43 | negative | positive | positive |
22 | Intestinal ln | 8 | no ct | no ct | no ct | no ct | no ct | no ct | negative | negative | negative |
Liver | 6 | no ct | no ct | no ct | no ct | no ct | no ct | ||||
Lung | 6 | no ct | no ct | no ct | no ct | – | – | ||||
Lung ln | 9 | no ct | no ct | no ct | no ct | – | – | ||||
Spleen | 6 | no ct | no ct | no ct | no ct | – | – | ||||
Kidney | 9 | no ct | no ct | no ct | no ct | – | – | ||||
Retropharyngeal lna | 5 | no ct | no ct | no ct | no ct | no ct | no ct | ||||
23 | Kidney | 2 | no ct | no ct | ne | ne | ne | ne | negative | positive | equivocal |
Muscle | 9 | no ct | no ct | 37.60 | 37.72 | 38.65 | 40.23 | ||||
Palatine tonsil | 3 | no ct | no ct | 39.57 | 41.83 | 39.17 | 39.14 | ||||
24 | Udder ln | 3 | no ct | no ct | no ct | no ct | no ct | no ct | negative | negative | negative |
25 | Intestinal ln | 2 | no ct | no ct | 32.92 | 34.22 | 31.61 | 31.05 | negative | positive | positive |
26 | Lung ln | 5 | no ct | no ct | no ct | no ct | – | – | negative | negative | negative |
Rumen | 5 | no ct | no ct | no ct | no ct | no ct | no ct | ||||
27 | Lung | 6 | no ct | no ct | no ct | no ct | – | – | negative | negative | negative |
Thymus | 16 | no ct | no ct | no ct | no ct | no ct | no ct | ||||
28 | Lung | 6 | no ct | no ct | 39.00 | no ct | – | – | negative | negative | – |
29 | Pelvic ln | 5 | no ct | no ct | no ct | no ct | – | – | negative | positive | positive |
Mesentery | 8 | no ct | no ct | 36.32 | 36.63 | 37.46 | 38.95 | ||||
Kidney | 6 | no ct | no ct | no ct | no ct | – | – | ||||
Perintoneum | 11 | no ct | no ct | no ct | no ct | no ct | no ct | ||||
30 | Intestinal ln | 3 | no ct | no ct | 34.14 | 34.49 | 38.22 | 38.39 | negative | positive | positive |
Liver ln | 5 | no ct | no ct | no ct | no ct | – | – | ||||
Lung ln | 6 | no ct | no ct | no ct | no ct | – | – | ||||
Retropharyngeal ln | 7 | no ct | no ct | no ct | no ct | – | – | ||||
31 | Kidney | 5 | no ct | no ct | no ct | no ct | negative | negative | – | ||
32 | Lung | 5 | no ct | no ct | no ct | no ct | no ct | no ct | negative | negative | negative |
Lung ln | 5 | no ct | no ct | no ct | no ct | – | – | ||||
33 | Lung ln | 6 | no ct | no ct | no ct | no ct | – | – | negative | negative | – |
34 | Spleen | 3 | no ct | no ct | no ct | no ct | no ct | no ct | negative | negative | negative |