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Table 2 Ct-values of the three DNA extraction protocols of the MTC-specific target genes Heli and IS 1081 generated with the field tissue samples of cattle

From: Two alternative DNA extraction methods to improve the detection of Mycobacterium-tuberculosis-complex members in cattle and red deer tissue samples

Animal No. Tissue Weight in grams Protocol 1 Protocol 2 Protocol 3 Protocol 1 Protocol 2 Protocol 3
Heli IS 1081 Heli IS 1081 Heli IS 1081 Overall result per animal
1 Lung 5 no ct no ct no ct no ct negative negative
Diaphragm 5 no ct no ct no ct no ct
2 Lung 5 no ct no ct no ct no ct negative negative
3 Intestinal ln 3 no ct no ct no ct no ct negative negative negative
Kidney 3 no ct no ct no ct no ct no ct no ct
Kidney ln 2 no ct no ct no ct no ct no ct no ct
4 Intestinal ln 5 no ct no ct no ct no ct negative negative negative
Liver 4 no ct no ct no ct no ct no ct no ct
5 Ln cervicalis superf. 8 no ct no ct no ct no ct no ct no ct negative negative negative
Mediastinal ln 10 no ct no ct no ct no ct no ct no ct
Kidney 7 no ct no ct no ct no ct
Kidney ln 4 no ct no ct no ct no ct
6 Liver 5 no ct no ct no ct no ct negative negative negative
Lung 2 no ct no ct no ct no ct no ct no ct
Kidney 4 no ct no ct no ct no ct
7 Lunga 3 no ct no ct no ct no ct no ct no ct negative negative
8 Lung ln 6 27.20 26.96 31.60 32.50 positive positive
9 Retropharyngeal ln 3 37.99 37.58 36.93 37.64 positive positive
10 Intestinal ln 4 31.10 30.54 28.87 29.32 31.85 32.08 positive positive positive
11 Liver ln 4 43.92 37.32 35.59 35.00 37.54 39.29 positive positive positive
Lung 2 34.26 31.18 37.77 39.37 37.56 38.68
12 Intestinal ln 5 28.21 26.69 31.10 31.50 positive positive
13 Intestinal ln 6 29.23 28.02 38.42 39.25 35.53 35.65 positive equivocal positive
14 Intestinal ln 4 no ct no ct 33.90 33.92 33.97 34.20 negative positive positive
15 Diaphragm 10 no ct no ct no ct no ct no ct no ct negative negative negative
Diaphragm 10 no ct no ct no ct no ct no ct no ct
Diaphragm 3 no ct no ct no ct no ct
16 Intestinal ln 3 35.31 35.60 no ct no ct 33.41 34.74 positive positive positive
Lung 3 no ct no ct no ct no ct no ct no ct
Lung ln 3 29.71 30.38 30.45 31.05 30.56 30.90
Spleen 6 no ct no ct no ct no ct 41.09 37.00
Kidney 8 no ct no ct no ct no ct no ct no ct
17 Intestinal ln 3 34.31 34.36 32.00 32.20 positive positive
18 Liver 4 no ct no ct no ct no ct positive positive positive
Lunga 5 26.53 27.10 24.80 24.86 26.28 26.73
Mediastinal ln 8 30.22 30.40 32.66 33.19 32.57 32.82
Spleen 5 no ct no ct 34.50 35.70
Kidney 3 no ct no ct 38.80 38.20
Retropharyngeal ln 2 no ct no ct ne ne
19 Mediastinal ln 3 no ct no ct no ct no ct no ct no ct negative negative negative
20 Intestinal ln 5 32.95 32.87 33.80 34.40 positive positive
Mediastinal ln 5 35.88 36.29 38.70 40.90
Retropharyngeal ln 4 no ct no ct no ct no ct
21 Lung ln 4 no ct no ct 29.29 30.08 31.59 31.43 negative positive positive
22 Intestinal ln 8 no ct no ct no ct no ct no ct no ct negative negative negative
Liver 6 no ct no ct no ct no ct no ct no ct
Lung 6 no ct no ct no ct no ct
Lung ln 9 no ct no ct no ct no ct
Spleen 6 no ct no ct no ct no ct
Kidney 9 no ct no ct no ct no ct
Retropharyngeal lna 5 no ct no ct no ct no ct no ct no ct
23 Kidney 2 no ct no ct ne ne ne ne negative positive equivocal
Muscle 9 no ct no ct 37.60 37.72 38.65 40.23
Palatine tonsil 3 no ct no ct 39.57 41.83 39.17 39.14
24 Udder ln 3 no ct no ct no ct no ct no ct no ct negative negative negative
25 Intestinal ln 2 no ct no ct 32.92 34.22 31.61 31.05 negative positive positive
26 Lung ln 5 no ct no ct no ct no ct negative negative negative
Rumen 5 no ct no ct no ct no ct no ct no ct
27 Lung 6 no ct no ct no ct no ct negative negative negative
Thymus 16 no ct no ct no ct no ct no ct no ct
28 Lung 6 no ct no ct 39.00 no ct negative negative
29 Pelvic ln 5 no ct no ct no ct no ct negative positive positive
Mesentery 8 no ct no ct 36.32 36.63 37.46 38.95
Kidney 6 no ct no ct no ct no ct
Perintoneum 11 no ct no ct no ct no ct no ct no ct
30 Intestinal ln 3 no ct no ct 34.14 34.49 38.22 38.39 negative positive positive
Liver ln 5 no ct no ct no ct no ct
Lung ln 6 no ct no ct no ct no ct
Retropharyngeal ln 7 no ct no ct no ct no ct
31 Kidney 5 no ct no ct no ct no ct    negative negative
32 Lung 5 no ct no ct no ct no ct no ct no ct negative negative negative
Lung ln 5 no ct no ct no ct no ct
33 Lung ln 6 no ct no ct no ct no ct negative negative
34 Spleen 3 no ct no ct no ct no ct no ct no ct negative negative negative
  1. ln lymph node, ne not evaluable
  2. – not done
  3. amicroscopic examination of Ziehl-Neelsen-stained sediments