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Table 2 Comparison of adenine in bead-beaten cell extracts with adenine in intact cellsa

From: A method for assessing efficiency of bacterial cell disruption and DNA release

Organism

Adenine in extracts of bead-beated cellsb (a)

Adenine in intact cellsb (b)

Extraction efficiency (a/b)

Mycobacterium smegmatis

299.0 +/− 22.4

394.0 +/− 5.4

75.9 %

Staphylococcus aureus

736.1 +/− 25.2

922.9 +/− 18.4

79.7 %

Francisella philomiragia

496.6+/− 10.2

597.1 +/− 20.0

83.1 %

Bacillus thuringiensis

179.6 +/− 12.4

258.6 +/− 1.0

69.5 %

Moraxella catarrhalis

299.0 +/− 18.0

394 +/− 5.4

75.9 %

Pseudomonas aeruginosa

501.0 +/− 20.0

624.9 +/− 4.9

80.2 %

  1. aAdenine released from DNA after acid/alkali treatment of extracts or intact cells (from approximately 109 cells), as described in Fig. 4
  2. bArea Under Curve of adenine peak (see Methods). Mean values +/− range of two separate samples are shown