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Table 2 Comparison of adenine in bead-beaten cell extracts with adenine in intact cellsa

From: A method for assessing efficiency of bacterial cell disruption and DNA release

Organism Adenine in extracts of bead-beated cellsb (a) Adenine in intact cellsb (b) Extraction efficiency (a/b)
Mycobacterium smegmatis 299.0 +/− 22.4 394.0 +/− 5.4 75.9 %
Staphylococcus aureus 736.1 +/− 25.2 922.9 +/− 18.4 79.7 %
Francisella philomiragia 496.6+/− 10.2 597.1 +/− 20.0 83.1 %
Bacillus thuringiensis 179.6 +/− 12.4 258.6 +/− 1.0 69.5 %
Moraxella catarrhalis 299.0 +/− 18.0 394 +/− 5.4 75.9 %
Pseudomonas aeruginosa 501.0 +/− 20.0 624.9 +/− 4.9 80.2 %
  1. aAdenine released from DNA after acid/alkali treatment of extracts or intact cells (from approximately 109 cells), as described in Fig. 4
  2. bArea Under Curve of adenine peak (see Methods). Mean values +/− range of two separate samples are shown