Fig. 9
Time-dependency of oxacillin plus α-mangostin-induced permeabilisation of the cytoplasmic membrane of ORSS-27055. The kinetics of α-mangostin-mediated ONPG passage across the cytoplasmic membrane are monitored as a change in the optical density (OD). The concentration of substrates was 100 μg/ml ONPG. CON = control (drugs free); OXA(64) = 64 μg/ml oxacillin; AMT(4) = 4 μg/ml α-mangostin; OXA(12) + AMT(1.5) = 12 μg/ml oxacillin plus 1.5 μg/ml α-mangostin; NIS(0.5) = 0.50 μg/ml nisin. Nisin at 0.50 μg/ml was used as a positive control, and untreated cells were used as a negative control. Each treatment performed two times in triplicate. The graph shows OD420nm of each treatment at the same time. Means sharing the same superscript at the same time are not significantly different from each other (Scheffe’s test, p < 0.01)