From: Diversity of Clostridium perfringens toxin-genotypes from dairy farms
Primer | Sequence (5′–3′) | Amplificationproduct size [bp] | Primer concentration [mM] | Toxin | Coding toxin gene |
---|---|---|---|---|---|
Multiplex PCR (major toxin genes)a | |||||
CPA5L | AGTCTACGCTTGGGATGGAA | 900 | 200 | alpha | cpa |
CPA5R | TTTCCTGGGTTGTCCATTTC | 200 | |||
CPBL | TCCTTTCTTGAGGGAGGATAAA | 611 | 400 | beta | cpb |
CPBR | TGAACCTCCTATTTTGTATCCCA | 400 | |||
CPIL | AAACGCATTAAAGCTCACACC | 293 | 200 | iota | iap |
CPIR | CTGCATAACCTGGAATGGCT | 200 | |||
CPETXL | TGGGAACTTCGATACAAGCA | 396 | 200 | epsilon | etx |
CPETXR | TTAACTCATCTCCCATAACTGCAC | 200 | |||
Duplex PCR (minor toxin genes)a | |||||
CPEL | GGGGAACCCTCAGTAGTTTCA | 506 | 400 | entero | cpe |
CPER | ACCAGCTGGATTTGAGTTTAATG | 400 | |||
CPB2L | CAAGCAATTGGGGGAGTTTA | 200 | 200 | beta-2 | cpb2 |
CPB2R | GCAGAATCAGGATTTTGACCA | 200 | |||
Singleplex PCR for cpa detectionb | |||||
224 | AGGAACTCATGCTATGATTGTAACTCAAGG | 775 | 200 | alpha | cpa |
972inverse | ACCACTAGTTGATATGTAAGCTACTAG | 200 | |||
PCR for cpb2 variantsc | |||||
CPB2ATYPF | ATTATGTTTAGGAATACAGTTA | 304 | 200 | beta-2 atypical | cpb2aty |
CPB2CONF | CAATTGGGGGAGTTTATCCACAA | 741 | 200 | beta-2 consensus | cpb2con |
CPB2R | CAATACCCTTCACCAAATACTC | 200 |