Fig. 6
The effect of B. cenocepacia on HLCC viability in 60 % ASMDM. Confluent HLCCs were exposed to 60 % ASMDM diluted in Ham’s F-12 complete medium, infected with B. cenocepacia at MOI 5 or mock-infected with saline, and incubated at 37 °C (7 % CO2). After 24 h, HLCC viability was determined using Trypan blue staining. There are statistically significant differences in mean cell viability between the different treatments (ANOVA, p < 0.0001). A Tukey-HSD post-hoc test indicated that the mean cell viability of infected HLCCs + 60 % ASMDM is significantly different than mock-infected HLCCs + Ham’s F-12 complete medium, infected HLCCs + Ham’s F-12 complete medium, and mock-infected HLCCs + 60 % ASMDM (p < 0.0001 for all). Values are averages of three independent experiments with single wells. Bars show standard deviation. Variables with different letters are statistically different (oneway ANOVA, p < 0.05)