Fig. 5
The effect of B. cenocepacia on HLCC density in 60 % ASMDM. Confluent HLCCs were exposed to 60 % ASMDM diluted in Ham’s F-12 complete medium, infected with B. cenocepacia at MOI 0.3-5 or mock-infected with saline, and incubated at 37 °C (7 % CO2). After 24 h, the cell density (cells/mL) of each well was compared to the cell density of T24 control HLCCs. T24 control consisted of HLCCs in Ham’s F-12 complete medium that were not manipulated (i.e. not washed, and medium not replaced) for the duration of the experiment. There are statistically significant differences in mean cell density ratio between the different treatments (ANOVA, p = 0.0037). A Tukey-HSD post-hoc test indicated that the mean cell density ratio in infected HLCCs + 60 % ASMDM is significantly different than in mock-infected HLCCs + Ham’s F-12 complete medium (p = 0.0055). The mean cell density ratio in mock-infected HLCCs + 60 % ASMDM is significantly different than in mock-infected HLCCs + Ham’s F-12 complete medium (p = 0.0135). Values are averages of three independent experiments with single wells. Bars show standard deviation. Variables with different letters are statistically different (oneway ANOVA, p < 0.05)