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Fig. 6 | BMC Microbiology

Fig. 6

From: The MarR-like protein PchR (YvmB) regulates expression of genes involved in pulcherriminic acid biosynthesis and in the initiation of sporulation in Bacillus subtilis

Fig. 6

Expression of various yvmB promoter luc fusions. a Structure of the yvmB operator region. The −10 and −35 regions similar to the consensus sequence of σA-dependent promoters are indicated: white boxes. The location of the cre site (Blencke et al. [34]) is indicated: grey box. Location of the palindromic sequence, palindrome I, is indicated in blue. Location of the palindromic sequence, palindrome II, is indicated in green. The different fusions with the luc gene are numbered with respect to the translational start site of yvmB. The −10 and −35 regions located in the yvmC promoter region are indicated: grey boxes. b Cells were grown in LB medium. Growth was monitored by measuring OD600: grey lines. Expression of the six pyvmB’-luc transcriptional fusions was measured in the wild-type: black lines. For each strain, one representative curve, out of three independent replicates realized, is shown. Note that the X-axe is different for the pF fusion to get a better view of the data. c Nucleotide sequence of the palindromic motif (green arrows), palindrome II. The nucleotides in bold belong to an inverted repeat nucleic sequence

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