Fig. 5From: The MarR-like protein PchR (YvmB) regulates expression of genes involved in pulcherriminic acid biosynthesis and in the initiation of sporulation in Bacillus subtilis Expression of various yvmC promoter lacZ fusions in wild-type and ΔyvmB strains. a Structure of the yvmC operator region. The −10 and −35 regions similar to the consensus sequence of σA-dependent promoters are indicated: white boxes. Location of the palindromic sequence, palindrome I, is indicated in blue. The DNA fragments used to construct pyvmC’-lacZ transcriptional fusions are diagrammed. They are numbered with respect to the translational start site of yvmC. Stars indicated the location of three point mutations introduced in the pA*yvmC’-lacZ fusion. The −10 and −35 regions located in the yvmB promoter region are indicated: grey boxes. b Cells were grown in LB medium. Growth was monitored by measuring OD600: grey line, wild-type; purple line, ΔyvmB. Expression of the four pyvmC’-lacZ transcriptional fusions was measured: black circles, in the wild-type; red squares, in ΔyvmB cells. Values are the means from at least three independent experiments. c Nucleotide sequence of the palindromic motif (blue arrows), palindrome I. The nucleotides in bold belong to the inverted repeat nucleic sequence. The A, C and A nucleotides at position −162, −163 and −164 (relative to the yvmC translational start site) were replaced by three nucleotides G to construct the pA*yvmC’-lacZ fusionBack to article page