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Fig. 5 | BMC Microbiology

Fig. 5

From: The MarR-like protein PchR (YvmB) regulates expression of genes involved in pulcherriminic acid biosynthesis and in the initiation of sporulation in Bacillus subtilis

Fig. 5

Expression of various yvmC promoter lacZ fusions in wild-type and ΔyvmB strains. a Structure of the yvmC operator region. The −10 and −35 regions similar to the consensus sequence of σA-dependent promoters are indicated: white boxes. Location of the palindromic sequence, palindrome I, is indicated in blue. The DNA fragments used to construct pyvmC’-lacZ transcriptional fusions are diagrammed. They are numbered with respect to the translational start site of yvmC. Stars indicated the location of three point mutations introduced in the pA*yvmC’-lacZ fusion. The −10 and −35 regions located in the yvmB promoter region are indicated: grey boxes. b Cells were grown in LB medium. Growth was monitored by measuring OD600: grey line, wild-type; purple line, ΔyvmB. Expression of the four pyvmC’-lacZ transcriptional fusions was measured: black circles, in the wild-type; red squares, in ΔyvmB cells. Values are the means from at least three independent experiments. c Nucleotide sequence of the palindromic motif (blue arrows), palindrome I. The nucleotides in bold belong to the inverted repeat nucleic sequence. The A, C and A nucleotides at position −162, −163 and −164 (relative to the yvmC translational start site) were replaced by three nucleotides G to construct the pA*yvmC’-lacZ fusion

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