Table 2 Primers used in this study

P330

GTCTGTGGTAGTTACTAGTTACTTTAAATACC

Forward primer for bbf27-30 target

P331

CCGAAATATTCCTATCTACTTAACAAC

Reverse primer for bbf27-30 target

P332

CCGGCCGGCGAATTTTGAGTCCTCTAGTGAGTTGTG

Left primer for inverse PCR of bbf27-30 target in pJET with NgoMIV site

P333

CCGGCTAGCGTTATAAGCCCTCCATTTGATAATTTTTTG

Right primer for inverse PCR of bbf27-30 target in pJET with NheI site

P366

CTTAATTTGTGACCGCCATTAGAGC

Forward primer for bbf27-30 screen

P367

GGGTTTTTTGAAACAAATCTTGC

Reverse primer for bbf27-30 screen

P411

GAGTTTCTGGTAAGATTAATGCTC

Forward primer for flaB RT-qPCR

P412

CATTTAAATTCCCTTCTGTTGTCTGA

Reverse primer for flaB RT-qPCR

P413

AGAGGTTTGTCACAAGCTTCTAGAAATACTTCAAAGGC

flaB RT-qPCR probe

P482

GAACAAGCTGAAAAATATAAAAAAGTAATG

Forward primer for PCR amplification of bbf19-22 target

P483

CTGGTTACTTTTTAGATAGAGTTTTTATAGAG

Reverse primer for PCR amplification of bbf19-22 target

P484

CCGGCCGGCGGTTTAGACTTGCATTTA TATCTCC

Left primer for inverse PCR bbf19-22 target in pJET with NgoMIV site

P485

CCGGCTAGCCCCCTCCTTATATTTTTTTATATATAAAAG

Right primer for inverse PCR of bbf19-22 target in pJET with NheI site

P486

GCTTATAAGCTTTATTAACACCCATATATTC

Forward primer for bbf19-22 screen

P487

CCCGCGAGGTATATTTATTTATATTG

Reverse primer for bbf19-22 screen

P709

TTACGGATTCTAATGCGGTTT

Forward primer for ospC RT-qPCR

P710

TTTACCAATAGCTTTAGCAGCAA

Reverse primer for ospC RT-qPCR

P711

TGTGAAAGAGGTTGAAGCGTTGCTG.

ospC RT-qPCR probe

P828

CTGCACTACCACAAGAGATTGCA

Forward primer for PCR screen for left-end sequence of lp28-1

P829

CTCTTCTCCTCTCTTCTTCTCTCT

Reverse primer for PCR screen for left-end sequence of lp28-1

P830

CATTTCTAGTCTAGATTGCAGTTATTTCTAAAATTAACT

Forward primer for PCR amplification for lp28-1 left-end deletion target

P831

GTGCCCAGGCGGCCGTCCTTATTCTTCTGGCATAGAAGT

Reverse primer for PCR amplification for lp28-1 left-end deletion target