Fig. 2

Generation and verification of lp28-1 mutant clones of B. burgdorferi. a Schematic of the construction strategy for the lp28-1 mutant clones Bb∆19-22 and Bb∆27-30 by allelic exchange is shown. Bb∆1-18 was generated by targeted telomere deletion as previously described in a published study [8]. To confirm the loss of regions bbf19-22 or bbf27-30, DNA was isolated and subjected to Southern blot analysis (b). All mutant clones provided a positive signal of the expected size (~28 kb) when probed for the kan cassette. The blot also confirmed the absence of the targeted region in the two mutant clones. WT, wild type; M, DNA size marker