Fig. 1
From: Bacillus anthracis S-layer protein BslA binds to extracellular matrix by interacting with laminin
Purification, and characterization of recombinant proteins. a Coomassie-stained SDS-PAGE of purified BslA(260–652). b Gram stain analysis of the function of BslA(260–652) in adherence of A16R to HeLa cells. a: pretreatment of B. anthracis A16R vegetative cell with naïve rabbit sera; b: pretreatment with anti-BslA(260–652) sera; c: pretreatment of HeLa cells with PBS; d: pretreatment with purified BslA(260–652). c Histogram of B. anthracis colony-forming units (cfu) extracted by lysis of host cells following infections pictured in (b). Error bars indicate one SEM. P-values were calculated using a two-tailed Student’s t-test