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Fig. 2 | BMC Microbiology

Fig. 2

From: Prevalence of adhesin and toxin genes in E. coli strains isolated from diarrheic and non-diarrheic pigs from smallholder herds in northern and eastern Uganda

Fig. 2

Electropherogram showing detection of virulence factors in E. coli isolates from diarrheic and non-diarrheic pigs. Lanes 1 and 25, 100 bp molecular weight marker (Bioron GmbH, Ludwigshafen, Germany). Lanes 2–4, 8–12,16–17, and 19 show E. coli DNA from the diarrheic pigs while lanes 5–7,13–15, and 18 show E. coli DNA from non-diarrheic pigs. Lanes 20, 21, 22 and 23, positive control DNA from E. coli isolates K88/NVI, Bd 3437/83 I, 853/67, and Bd 60/84 I, respectively. Lane 24, negative control consisting of a blank sample without DNA. The black arrows from top to bottom show the positions for F4 (601 bp), F6 (333 bp), LT (236 bp), STa (160 bp) and STb (114 bp). The PCR amplicons were electrophoresed on a 2% agarose gel stained with SYBR® safe DNA gel stain and visualized under UV-transillumination

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