Table 2 Virulence and virulence-associated genes in C. perfringens strain CP1 that were significantly expressed under different conditions
| Â | Â | Â | in vivo-RM | in vivo-PM | in vivo-OS | PM-RM | OS-RM | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Feature | Locus Tag | Predict product (gene name) | Fold | log2FC | FDR | Fold | log2FC | FDR | Fold | log2FC | FDR | Fold | log2FC | FDR | Fold | log2FC | FDR |
V | CP4_0080 | phospholipase C (plc) | 9.3 | 3.2 | 0.0 | Â | Â | Â | 7.1 | 2.8 | 0.0 | 6.9 | 2.8 | 0.0 | Â | Â | Â |
V | CP4_0193 | peptidoglycan protein (cna) | −8.5 | −3.1 | 0.0 |  |  |  |  |  |  |  |  |  | −7.5 | −2.9 | 0.0 |
V | CP4_0211 | collagenase_kappa toxin (colA) | 4.7 | 2.2 | 0.0 | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â |
V | CP4_0212 | collagenase_kappa toxin (colA) | 4.9 | 2.3 | 0.0 | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â |
QS | CP4_0214 | UbiE/COQ5 family methlytransferase (ycgJ) | −59.4 | −5.9 | 0.0 |  |  |  |  |  |  | −22.5 | −4.5 | 0.0 | −112.0 | −6.8 | 0.0 |
QS | CP4_0215 | cystathionine beta-lyase (metB) | −59.3 | −5.9 | 0.0 |  |  |  |  |  |  | −18.6 | −4.2 | 0.0 | −40.2 | −5.3 | 0.0 |
QS | CP4_0216 | cysteine synthase (cysK) | −30.7 | −4.9 | 0.0 |  |  |  |  |  |  | −17.8 | −4.2 | 0.0 | −31.5 | −5.0 | 0.0 |
QS | CP4_0217 | S-ribosylhomocysteinase (luxS) | −27.3 | −4.8 | 0.0 |  |  |  |  |  |  | −7.2 | −2.9 | 0.0 | −29.8 | −4.9 | 0.0 |
NELoc-2 | CP4_0460 | putative VTC domain superfamily |  |  | 0.0 |  |  |  | −5.8 | −2.5 | 0.0 |  |  |  |  |  |  |
NELoc-2 | CP4_0461 | putative tubulin/FtsZ, GTPase | −6.3 | −2.7 | 0.0 | −5.4 | −2.4 | 0.0 | −8.3 | −3.1 | 0.0 |  |  |  |  |  |  |
VR10B | CP4_0572 | collagen adhesin (cnaA) |  |  | 0.0 |  |  |  | −7.4 | −2.9 | 0.0 |  |  |  |  |  |  |
VR10B | CP4_0573 | signal peptidase I (lepB) | −5.3 | −2.4 | 0.0 |  |  |  | −11.0 | −3.5 | 0.0 | −4.2 | −2.1 | 0.0 |  |  |  |
VR10B | CP4_0574 | PitB | −4.6 | −2.2 | 0.0 |  |  |  | −9.2 | −3.2 | 0.0 |  |  |  |  |  |  |
VR10B | CP4_0575 | sortase, SrtB family (srtB) | −4.1 | −2.0 | 0.0 |  |  |  | −8.7 | −3.1 | 0.0 | −4.3 | −2.1 | 0.0 |  |  |  |
VR10B | CP4_0576 | putative streptococcal pilin isopeptide link | −4.2 | −2.1 | 0.0 |  |  |  | −9.8 | −3.3 | 0.0 |  |  |  |  |  |  |
V | CP4_0806 | sialidase (nanI) | 76.1 | 6.3 | 0.0 | Â | Â | Â | 129.2 | 7.0 | 0.0 | 132.8 | 7.1 | 0.0 | Â | Â | Â |
regulator | CP4_0922 | VirT (virT) | 30.1 | 4.9 | 0.0 | Â | Â | Â | 32.4 | 5.0 | 0.0 | 78.1 | 6.3 | 0.0 | Â | Â | Â |
regulator | CP4_1328 | VR-RNA (vrr) |  |  | 0.0 |  |  |  | −16.1 | −4.0 | 0.0 | −5.6 | −2.5 | 0.0 | 4.2 | 2.1 | 0.0 |
V | CP4_1570 | enterotoxin (entD) | 4.0 | 2.0 | 0.0 | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â |
V | CP4_1581 | hyaluronidase _mu-toxin (nagJ) | 4.9 | 2.3 | 0.0 | Â | Â | Â | 13.0 | 3.7 | 0.0 | 5.0 | 2.3 | 0.0 | Â | Â | Â |
V | CP4_2582 | hyaluronidase _mu-toxin (nagA) | Â | Â | 0.0 | Â | Â | Â | Â | Â | Â | 8.2 | 3.0 | 0.0 | Â | Â | Â |
plasmid | CP4_3441 | beta2 toxin (cpb2) | Â | Â | Â | Â | Â | Â | 8.3 | 3.1 | 0.0 | 6.9 | 2.8 | 0.0 | Â | Â | Â |
NELoc-1 | CP4_3443 | beta-lactamase domain-containing protein |  |  | 0.3 | 6.7 | 2.7 | 0.0 |  |  |  | −4.9 | −2.3 | 0.0 |  |  |  |
NELoc-1 | CP4_3444 | M protein trans-regulator | −15.7 | −4.0 | 0.0 | −4.6 | −2.3 | 0.3 | −24.3 | −4.6 | 0.0 |  |  |  |  |  |  |
NELoc-1 | CP4_3445 | SAM domain-containing protein | −14.0 | −3.8 | 0.0 | −22.7 | −4.5 | 0.0 | −14.4 | −3.9 | 0.0 |  |  |  |  |  |  |
NELoc-1 | CP4_3446 | putative internalin | −8.5 | −3.1 | 0.1 | −8.0 | −3.0 | 0.1 | −20.2 | −4.3 | 0.0 |  |  |  |  |  |  |
NELoc-1 | CP4_3454 | chitinase B | 19.6 | 4.3 | 0.0 | Â | Â | Â | 70.3 | 6.1 | 0.0 | 26.9 | 4.8 | 0.0 | Â | Â | Â |
NELoc-1 | CP4_3455 | chitodextrinase | 31.1 | 5.0 | 0.0 | Â | Â | Â | 112.2 | 6.8 | 0.0 | 39.2 | 5.3 | 0.0 | Â | Â | Â |
NELoc-1 | CP4_3458 | pore-forming toxin_NetB (part) |  |  | 0.0 | 28.2 | 4.8 | 0.0 |  |  |  | −8.5 | −3.1 | 0.0 |  |  |  |
NELoc-1 | CP4_3459 | pore-forming toxin_NetB (part) | 4.2 | 2.1 | 0.0 | 29.9 | 4.9 | 0.0 | 4.5 | 2.2 | 0.0 | −7.1 | −2.8 | 0.0 |  |  |  |
NELoc-1 | CP4_3460 | hypothetical protein | −12.9 | −3.7 | 0.0 |  |  |  |  |  |  |  |  |  | −4.5 | −2.2 | 0.0 |
NELoc-1 | CP4_3461 | hypothetical protein | −4.2 | −2.1 | 0.0 |  |  |  | −6.1 | −2.6 | 0.0 |  |  |  |  |  |  |
NELoc-1 | CP4_3464 | resolvase/recombinase |  |  | 0.0 |  |  |  |  |  |  | −7.7 | −3.0 | 0.0 |  |  |  |
NELoc-1 | CP4_3465 | resolvase/recombinase |  |  | 0.0 |  |  |  |  |  |  | −7.5 | −2.9 | 0.0 |  |  |  |
NELoc-1 | CP4_3475 | ABC transporter |  |  | 0.0 |  |  |  |  |  |  | −5.7 | −2.5 | 0.0 |  |  |  |
NELoc-1 | CP4_3478 | diguanylate cyclase/phosphodiesterase | −4.1 | −2.0 | 0.0 | −4.9 | −2.3 | 0.0 | −6.5 | −2.7 | 0.0 |  |  |  |  |  |  |
NELoc-3 | CP4_3569 | resolvase/recombinase | Â | Â | Â | Â | Â | Â | 5.8 | 2.5 | 0.0 | Â | Â | Â | Â | Â | Â |
NELoc-3 | CP4_3570 | hypothetical protein | −5.8 | −2.5 | 0.1 |  |  |  |  |  |  |  |  |  | −4.4 | −2.2 | 0.0 |