Table 2 Plasmids used in this study

pBRDX

Suicide delivery vector, rdxA sacB Cm

[54]

pBRΔclpP

pBRDX::clpP for clpP deletion

This study

pJB908

Insert thy gene, mutate mob into pkB5

[4]

pBC(gfp)Pmip

ColE1 ori Cm Pmip gfpmut2

[59]

pZL01

Insert SacI/PstI fragment encoding GFP, Pmip into pJB908

This study

pXL901

Complementation plasmid, derived from pZL01, with gfpmut2 changed for clpP

This study

pTLpflaG

Insert BamHI/SphI fragment encoding GFP, flaA promoter into pTLP6

[57]

pGB908

Insert XbaI/SphI fragment of gfpmut3 into pJB908

This study

pGB908X*

Insert 9 icm promoters of operon areas into pGB908

This study

pCyaSidJ

Insert EcoRI/SalI fragment of CyaA catalytic domain and SidJ into pKB5

[34, 50]