Fig. 6

clpP-deficiency impaired the translocation of some effectors. J774A.1 cells were infected with the wild type (Lp02), the dotA mutant (Lp03) and the clpP mutant (Xp02) L. pneumophila strains expressing the indicated Cya hybrid proteins. After 1 h of infection, tissue culture cells were lysed and cAMP was extracted from the sample. Total cAMP production induced by translocation of the hybrid was quantified using an enzyme-immunoassay system, indicated as pmol. Results represent average values of experiments performed in triplicate. **, p < 0.01