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Fig. 2 | BMC Microbiology

Fig. 2

From: MicroRNA-125b-5p suppresses Brucella abortus intracellular survival via control of A20 expression

Fig. 2

MiR-125b-5p downregulates its target A20. a RAW264.7 cells were treated with or without TNFα (10 ng/ml). b RAW264.7 cells, transduced with lentivirus containing scrambled microRNA (Scr-miR) or pre-miR-125b-5p, were treated with TNFα (10 ng/ml) for 4 h. Cell lysates a and b were subjected to immunoblotting analysis of A20. c RAW264.7 cells, transduced with lentivirus containing Scr-miR or pre-miR-125b-5p, were treated with 1000 ng/ml E. coli LPS (ELPS) for the indicated time points followed by immunoblotting of the cell lysates for IkBα and β-actin Western analysis. The blot is a representative of 3 independent experiments. The quantitative data for A20 and IkBα expression are shown under Western blotting data. Data are expressed as mean ± SEM (n = 3). The maximal ratio of IkBα or A20 expression /β-actin in the group was defined as 100 % (or control). *P < 0.05 VS control

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