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Table 2 Evaluation of E. coli expression vectors

From: Recombinant expression of Chlamydia trachomatis major outer membrane protein in E. Coli outer membrane as a substrate for vaccine research

Vector

Promoter/Strength

Inducer

Origin of Replication

Copy Number

rCtE-MOMP Surface Expression (GFIa)

bpAVE029

λPL/Moderate

IPTG

pAT153(colE1)

Low

Good (~300)

pACYDuet-1

T7/Titratable

Arabinose + IPTG

p15A

Low

Intermediate (~120)

pET (pETBlue-1 and pET22b)

T7/Strong

IPTG

pUC

High

Low (~30 or lower)

pWSK29

T7/T3/Strong

IPTG

pSC101

Low

None (intracellular)

pJ831(pUC)

T7/Titratable

Rhamnose

pMB1

High

None (intracellular)

pJ841(pBR)

T7/Titratable

Rhamnose

pMB1

Medium

None (intracellular)

pJ851(pACYC)

T7/Titratable

Rhamnose

p15A

Low

None (intracellular)

  1. aGFI: geomean fluorescence intensity from whole cell flow cytometry antibody binding assay
  2. bpAVE029 is an E. coli RNA polymerase dependent expression vector. Others listed are bacteriophage T7 RNA polymerase dependent expression vectors