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Table 1 Activation of human macrophages migration induced by Pet Protein in a Boyden chamber assay

From: Pet serine protease from enteroaggregative Escherichia coli stimulates the inflammatory response activating human macrophages

Treatment

Cells number × fieldsa

Macrophages migration

percentage

Hank’s solution BSA (0.2 %)

5 ± 2.5

16

ZAS

30 ± 3.4

100

Pet 5 μg/ml

10 ± 2.2

33

Pet 10 μg/ml

15 ± 5.1

50

Pet 20 μg/ml

22 ± 4.3

73

  1. Values are mean ± SD
  2. aCells x fields refers the migrated cells counted in five randomly selected fields what were observed under a microscope set at 400 X magnification. The date of percentage presented are compared with the 30 cells observed with the stimulus treatment with ZAS (Positive control)