Fig. 3From: Pet serine protease from enteroaggregative Escherichia coli stimulates the inflammatory response activating human macrophagesCytokines production of MDM stimulated with Pet toxin from EAEC. Supernatants of MDM stimulated with Pet (1 to 20 μg/ml) were analyzed by ELISA test to determine the cytokines expression. a IL-8, b TNF-α and c IL-10. The MDM cells were treated with E. coli. 0111:B4 LPS (0.1 μg/ml); Polymyxin B (PMB), LPS and Polymyxin B (LPS/PMB), Pet (20 μg/ml) and Polymyxin B (Pet20/PMB), and untreated cells (s/e). The cytokine concentrations weredeterminedat 6(□) and 24 h (■) of stimulation. The results are the means ± of the standard deviation representative of four independent experiments. Significant P < 0.05 (*), as compared between 6 and 24 h of stimulationBack to article page