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Fig. 3 | BMC Microbiology

Fig. 3

From: Pet serine protease from enteroaggregative Escherichia coli stimulates the inflammatory response activating human macrophages

Fig. 3

Cytokines production of MDM stimulated with Pet toxin from EAEC. Supernatants of MDM stimulated with Pet (1 to 20 μg/ml) were analyzed by ELISA test to determine the cytokines expression. a IL-8, b TNF-α and c IL-10. The MDM cells were treated with E. coli. 0111:B4 LPS (0.1 μg/ml); Polymyxin B (PMB), LPS and Polymyxin B (LPS/PMB), Pet (20 μg/ml) and Polymyxin B (Pet20/PMB), and untreated cells (s/e). The cytokine concentrations weredeterminedat 6(□) and 24 h (■) of stimulation. The results are the means ± of the standard deviation representative of four independent experiments. Significant P < 0.05 (*), as compared between 6 and 24 h of stimulation

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