Fig. 6
Detection of ClpBLi in hamster kidney tissues. The macerated kidney tissues containing ~20 μg proteins were subjected to SDS-PAGE followed by Western blotting with anti-ClpBLi158–334 serum. Lane 1, purified ClpBLi (a positive control); lanes 2, 3, 5, 6, the kidney homogenates from hamsters infected with L. interrogans serovar Hardjo and euthanized 14 (lane 2) or 6 (lanes: 3,5,6) weeks post infection; lane 4, the kidney homogenate from an uninfected hamster. The arrow indicates the position of the 6His-tagged ClpBLi (~98.5 kDa). The position of 100-kDa protein marker (PageRuler unstained Protein Ladder; Thermo Scientific), is shown on the ... left