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Fig. 2 | BMC Microbiology

Fig. 2

From: Immunoreactivity of the AAA+ chaperone ClpB from Leptospira interrogans with sera from Leptospira-infected animals

Fig. 2

Purification of L. interrogans ClpB. a The Coomassie blue-stained SDS-PAGE gel showing the lysates from E. coli cells transformed with the recombinant plasmid expressing clpB Li (pET28clpB Li) without induction (−) (lane 1) and induced with IPTG (+) (lane 2), and the representative fractions obtained following the nickel resin (Ni-NTA, lane 3) and gel filtration (GF, lane 4) purification of ClpBLi. The arrow indicates the position of the 6His-tagged ClpBLi (~98.5 kDa). b The Coomassie blue-stained SDS-PAGE gel showing ClpBLi digested with thrombin (lane 1) and the 6His-tagged ClpBLi (lane 2). The positions of protein size markers (M) (in kDa), PageRuler prestained Protein Ladder (Thermo Scientific), are shown on the ... left

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