Bacteroides fragilis metabolises exopolysaccharides produced by bifidobacteria

Rios-Covian, David; Cuesta, Isabel; Alvarez-Buylla, Jorge R.; Ruas-Madiedo, Patricia; Gueimonde, Miguel; de los Reyes-Gavilán, Clara G.

doi:10.1186/s12866-016-0773-9

Table 1 Growth and metabolic parameters of B. fragilis grown in glucose, EPS or without carbon source

From: Bacteroides fragilis metabolises exopolysaccharides produced by bifidobacteria

Carbon source	Time (h)	Acetic acid	Propionic acid	Lactic acid	Succinic acid	Formic acid	Total metabolites*	Acetic/Propionic	Propionic/Succinic	Acetic/Lactic	Bacterial counts⁺
Glucose	24	13.00 ± 0.41^D	4.35 ± 0.63^C	14.81 ± 0.27^B	3.46 ± 0.19^{a B}	7.33 ± 0.15^D	42.95 ± 1.40^{a C}	3.02 ± 0.35^C	1.25 ± 0.12^A	0.88 ± 0.01^A	9.58 ± 0.07^{d C}
	48	13.28 ± 0.74^C	4.69 ± 0.21^C	17.11 ± 1.26^B	3.75 ± 0.27^{a B}	6.86 ± 0.38^D	45.73 ± 1.22^{ab D}	2.83 ± 0.08^D	1.25 ± 0.13^A	0.78 ± 0.07^A	8.47 ± 0.16^{c B}
	72	13.80 ± 0.53^C	5.04 ± 0.47^D	16.15 ± 1.01^B	4.37 ± 0.18^{b C}	7.59 ± 0.12^C	47.11 ± 0.41^{b C}	2.75 ± 0.18^D	1.15 ± 0.09^A	0.86 ± 0.09	7.76 ± 0.06^{b A}
	144	13.66 ± 0.26^D	4.96 ± 0.32^D	15.27 ± 0.87^B	4.56 ± 0.52^{b B}	7.26 ± 0.45^C	46.02 ± 1.96^{ab D}	2.76 ± 0.15^C	1.09 ± 0.06^A	0.90 ± 0.05^A	7.46 ± 0.17^{a B}
EPS E44	24	1.24 ± 0.06^{b B}	0.78 ± 0.08^{b A,B}	0.45 ± 0.07^{b A}	0.12 ± 0.02^A	0.43 ± 0.04^{a B}	3.01 ± 0.11^{a B}	1.60 ± 0.10^{a B}	6.78 ± 0.27^D	2.83 ± 0.53^{a A}	9.17 ± 0.12^{c B}
	48	0.93 ± 0.08^{a A,B}	0.39 ± 0.08^{a A}	0.95 ± 0.05^{c A}	-	1.27 ± 0.24^{b C}	3.56 ± 0.22^{b C}	2.44 ± 0.30^{b C}	ND	0.98 ± 0.12^{a A}	8.91 ± 0.02^{b C}
	72	1.89 ± 0.10^{c B}	0.95 ± 0.13^{b B}	0.77 ± 0.08^{c A}	-	1.67 ± 0.46^{b B}	5.31 ± 0.34^{c B}	2.01 ± 0.19^{ab C}	ND	2.48 ± 0.38^a	9.05 ± 0.05^{bc B}
	144	5.53 ± 0.08^{d C}	2.75 ± 0.13^{c C}	0.26 ± 0.15^{a A}	0.13 ± 0.05^A	1.40 ± 0.31^{b B}	10.07 ± 0.39^{d C}	2.01 ± 0.12^{ab B}	25.22 ± 11.55^B	26.24 ± 12.29^{b A}	8.68 ± 0.14^{a C}
EPS R1	24	1.70 ± 0.11^{a C}	1.37 ± 0.12^{b B}	0.16 ± 0.10^{b A}	0.26 ± 0.05^{b A}	0.73 ± 0.25^{a C}	4.22 ± 0.09^{a B}	1.25 ± 0.03^{a B}	5.40 ± 0.54^{b C}	14.03 ± 7.32^{a B}	9.01 ± 0.09^{b B}
	48	1.40 ± 0.22^{a B}	0.82 ± 0.12^{a B}	0.49 ± 0.09^{c A}	0.15 ± 0.01^{a A}	1.92 ± 0.12^{c B}	4.81 ± 0.37^{b B}	1.70 ± 0.08^{b B}	5.64 ± 0.55^{b B}	2.86 ± 0.12^{a B}	9.17 ± 0.09^{b D}
	72	2.08 ± 0.10^{b B}	1.44 ± 0.16^{b C}	0.12 ± 0.09^{b A}	0.26 ± 0.00^{b B}	1.35 ± 0.18^{b B}	5.25 ± 0.09^{b B}	1.45 ± 0.11^{a B}	5.62 ± 0.62^{b B}	25.26 ± 19.65^a	9.15 ± 0.13^{b B}
	144	3.13 ± 0.18^{c B}	1.70 ± 0.09^{c B}	0.02 ± 0.01^{a A}	0.53 ± 0.02^{c A}	1.27 ± 0.26^{b B}	6.65 ± 0.34^{c B}	1.85 ± 0.20^{b B}	3.21 ± 0.29^{a A}	>100^{b B}	6.73 ± 0.21^{a A}
WCS	24	0.21 ± 0.05^{a A}	0.26 ± 0.03^{a A}	-	0.11 ± 0.07^{a A}	0.09 ± 0.03^{a A}	0.72 ± 0.12^{a A}	0.79 ± 0.12^A	2.33 ± 0.43 ^B	ND	8.36 ± 0.15^{c A}
	48	0.27 ± 0.05^{a A}	0.34 ± 0.03^{b A}	-	0.21 ± 0.01^{b A}	0.17 ± 0.03^{b A}	1.01 ± 0.05^{a A}	0.79 ± 0.10^A	1.66 ± 0.21^A	ND	8.10 ± 0.06^{c A}
	72	0.35 ± 0.06^{a A}	0.36 ± 0.00^{b A}	-	0.20 ± 0.03^{b A}	0.17 ± 0.04^{b A}	1.08 ± 0.02^{a A}	0.99 ± 0.16^A	1.83 ± 0.29 ^A	ND	7.84 ± 0.11^{b A}
	144	0.66 ± 0.22^{b A}	0.51 ± 0.05^{c A}	-	0.23 ± 0.05^{b A}	0.26 ± 0.03^{c A}	1.68 ± 0.35^{b A}	1.28 ± 0.30^A	2.30 ± 0.36 ^A	ND	6.61 ± 0.19^{a A}

Metabolite concentrations are expressed in mM, bacterial counts are expressed in ufc mL⁻¹ Columns for the same carbon source with different lower case letter superscripts indicate significant differences along time (p > 0.05). Different capital letters superscripts indicate significant differences among carbon sources at the same sampling point (p < 0.05). * Sum of acetic, propionic, lactic, succinic, formic and pyruvic acids. ⁺ Counts at 0 h were 7.02 ± 0.08 log ufc mL⁻¹. WCS without carbon source. -, under the detection limit, ND Not determined

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