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Fig. 3 | BMC Microbiology

Fig. 3

From: RsmW, Pseudomonas aeruginosa small non-coding RsmA-binding RNA upregulated in biofilm versus planktonic growth conditions

Fig. 3

RsmW transcription is elevated in stationary phase and in gacA and rhlR deficient backgrounds. P. aeruginosa strains were grown in 5 ml cultures at 37 °C with 250 rpm shaking. Aliquots were processed and placed in a 96-well microtiter plate for absorbance readings at 420 nm as previously described by Griffith et al. [73]. The y-axis unit represents β-galactosidase activity normalized by OD600 nm of the cultures. To account for leaky expression or background noise arising from the strains or fusion system β-galactosidase activity from experimental strains was subtracted from a parental strain harboring an empty transcriptional fusion construct. All numbers indicate the average of three independent experiments. Error bars indicate mean ± standard error (s.e.m.). a Schematic representation of transcriptional fusions integrated into the chromosomes of wild-type and rhlR and gacA transposon mutants. b RsmW transcription is elevated in minimal media during stationary growth phase. P. aeruginosa strains were grown in minimal (PB) or nutrient rich (LB) media and samples at mid-logarithmic (OD600 nm = 0.6) and stationary phase (16 h) were analyzed for lacZ expression using both the rsmWS-lacZ and rsmWL-lacZ fusions. c RsmW transcription is elevated 2-fold in rhlR transposon mutant in minimal media during stationary growth phase. P. aeruginosa rhlR transposon mutants were grown in minimal (PB) or nutrient rich (LB) media and samples at stationary phase (16 h) were collected and analyzed using the rsmWL-lacZ fusion. d RsmW transcription is induced 27-fold in gacA transposon mutant. P. aeruginosa gacA transposon mutants grown in minimal (PB) media at mid-logarithmic (OD600 nm = 0.6) and stationary phase (16 h) were analyzed using the rsmWL-lacZ fusion. Abbreviations: log (mid-logarithmic phase); ON (stationary phase (16 hours))

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