Consensus computational network analysis for identifying candidate outer membrane proteins from Borrelia spirochetes

Table 3 Oligonucleotides utilized in the study

Primer Name	Sequence 5’-3’^a	Description
BB0838 (2362) F	GCGGCTAGCTTATCTGATCCGGAAACTTTTTA	Cloning bb0838 (2362) into the pET23a vector
BB0838 R	GCGCTCGAGTCTATTAATAATAAACTCGTAGTTT	Cloning bb0838 (2362) into the pET23a vector
BB0405 F	GCGGCTAGCTCCAAAAGCAAAAGTATGACTG	Cloning bb0405 into the pET23a vector
BB0405 R	GCGCTCGAGTATATATATTTTTATAAAGCCTGTG	Cloning bb0405 into the pET23a vector
BB0406 F	GCGGGATCCTCTTTTGCATCTGACAATTATATG	Cloning bb0406 into the pET23a vector
BB0406 R	GCGCTCGAGTGCAAATTTTATGAATCCAAATCC	Cloning bb0406 into the pET23a vector
BB0838 RT 3’	ATCTTTAGTAAGTCCATAAGTGAAATTTT	cDNA synthesis for uvrA-bb0838 PCR reaction
UvrA RT 3’	GAGCCACTCTTGCCAGATATTA	cDNA synthesis for uvrB-uvrA PCR reaction
BB0406 RT 3’	AATTCTTATAACAGCGCCTATTCTCTCATA	cDNA synthesis for bb0405-bb0406 PCR reaction
BB0405 RT 3’	CATAGTTGTTCCAATAGTAGCAACAGC	cDNA synthesis for bb0404-bb0405 PCR reaction
UvrB PCR 5’	GATTGTCTAAAAAAAAGCTTATTGATAAG	RT-PCR of uvrB-uvrA
UvrA PCR 3’	TGGAATATCTACATCAACATTTTTTAAATT	RT-PCR of uvrB-uvrA
UvrA PCR 5’	GTTTCTGGTATTCCTGAAGAGG	RT-PCR of uvrA-bb0838
BB0838 PCR 3’	CCAGATCCGGCAAGTCCC	RT-PCR of uvrA-bb0838
BB0404 PCR 5’	ATTAATGGCCTAAAGTTAGCTTCAAAAAG	RT-PCR of bb0404-bb0405
BB0405 PCR 3’	GCTGTACTCTATTACCAAAGGCAA	RT-PCR of bb0404-bb0405
BB0405 PCR 5’	GTTGTGATGGGTGTAGATCTTCT	RT-PCR of bb0405-bb0406
BB0406 PCR 3’	AATTCTTATAACAGCGCCTATTCTCTCATA	RT-PCR of bb0405-bb0406

ISSN: 1471-2180