Consensus computational network analysis for identifying candidate outer membrane proteins from Borrelia spirochetes

Table 1 Borrelia burgdorferi B31 candidate OMPs

Protein	Outer membrane localization/β-barrel conformation^a						Signal sequence^b
Protein	CELLO	PSORTb	HHOMP	TMBETADISC -ACC	PRED-TMBB	BOMP	SignalP 3.0	PrediSI	Signal-CF	Kyte Doolittle
Group 1 (6/6)
BB0418 (DipA)	+	+	+	+	+	+	+	+	+	+
BB0794	+	+	+	+	+	+	-	+	+	+
BB0795 (BamA)	+	+	+	+	+	+	-	+	+	+
BB0838	+	+	+	+	+	+	+	+	+	+
Group 2 (5/6)
BB0110	+	+	+	+	+	-	-	-	-	+
BB0236	+	+	-	+	+	+	-	+	-	+
BB0603 (P66)	+	+	+	+	+	-	+	+	+	+
BB0667	+	+	-	+	+	+	-	+	-	+
BB0811	+	+	-	+	+	+	+	+	+	+
BB0824	+	-	+	+	+	+	-	+	+	+
Group 3 (4/6)
BB0027	+	-	+	+	+	-	-	-	+	+
BB0089	+	+	-	+	-	+	+	+	+	+
BB0142 (BesC)	+	-	+	+	+	-	-	-	-	+
BB0156	+	+	-	+	+	-	-	-	+	+
BB0159	+	+	-	+	+	-	+	-	-	+
BB0161	+	+	+	-	-	+	-	-	-	+
BB0308	+	+	-	+	-	+	-	+	+	+
BB0319	+	+	-	+	-	+	-	-	-	+
BB0464	+	+	-	+	-	+	+	+	+	+
BB0465	+	+	+	-	+	-	-	+	+	+
BB0543	-	+	+	+	+	-	-	+	+	+
BB0564	-	+	+	+	+	-	-	-	-	+
BB0624	+	+	-	+	+	-	-	-	-	+
BB0662	+	+	-	+	+	-	-	+	-	+
BB0743	+	+	-	+	-	+	-	+	-	+
BB0761	+	+	-	+	-	+	-	-	-	+
Group 4 (3/6)
BB0032	+	+	-	+	-	-	-	-	+	+
BB0039	+	-	-	+	-	+	-	+	+	+
BB0043	+	-	-	+	+	-	-	-	+	+
BB0058	+	-	-	+	-	+	+	+	+	+
BB0102	+	+	-	+	-	-	+	+	-	-
BB0125	+	+	-	+	-	-	-	-	+	-
BB0165	+	+	-	+	-	-	-	-	-	+
BB0322	+	+	+	-	+	-	-	-	-	+
BB0352	+	+	-	+	-	-	+	+	-	+
BB0405	-	+	+	-	+	-	-	+	+	+
BB0406	+	+	+	-	-	-	+	+	-	+
BB0458	+	+	-	+	-	-	-	+	-	+
BB0546	+	+	-	+	-	-	-	-	+	+
BB0735	+	+	-	+	-	-	-	+	+	+
BB0790	+	-	-	+	-	+	-	-	+	+

^aProteins are grouped according to the number of OM prediction programs that predict localization to the OM or β-barrel formation. The outer membrane protein prediction programs included CELLO, PSORTb, HHOMP, TMBETADISC-ACC, PRED-TMBB and BOMP
^bSignal sequences were predicted by the programs SignalP 3.0, PrediSi, and Signal-CF. Signal sequences were also manually inspected by hydrophilicity plots generated according to the methods of Kyte and Doolittle

ISSN: 1471-2180