a. Strains and growth conditions | Â | Â | Â |
---|---|---|---|
Strain | Origin | Agar/Broth | Growth conditions |
Lactobacillus rhamnosus GG ATCC 53103 (L.GG) | Valio Ltd., Helsinki, Finland | de Man, Rogosa and Sharpe (MRS) | 24 h, 37 °C, 5 % CO2 |
Candida albicans ATCC 10231 (Ca) | American Type Culture Collection (ATCC) | Sabouraud | 24 h, 37 °C, air |
Streptococcus mutans ATCC 27351 (Sm) | ATCC | Brain Heart Infusion (BHI) | 24 h, 37 °C, 5 % CO2 |
Streptococcus sanguinis ATCC 10556 (Ss) | ATCC | BHI | 24 h, 37 °C, 5 % CO2 |
Aggregatibacter actinomycetemcomitans ATCC 43718 (Aa) | ATCC | BHI | 24 h, 37 °C, 5 % CO2 |
Fusobacterium nucleatum ATCC 25586 (Fn) | ATCC | Brucella | 48 h, 37 °C, in anaerobic condition (mixture of 0.2 % O2, 5 % CO2, 9.9 % H2, 84.9 % N2) |
b. Group setup of dual- and multi- species biofilm groups and respective agars and cultural conditions to detect viable cells from biofilms | |||
Groups | Strain(s) | Agars and cultural conditions | |
L.GG + Ca | L.GG, Ca | 72 h, 37 °C, L.GG: MRS in 5 % CO2 condition Ca: Sabouraud in air Sm, Ss, Aa, Fn: BHI in anaerobic condition | |
L.GG + Sm | L.GG, Sm | ||
L.GG + Ss | L.GG, Ss | ||
4SP | Ss, Aa, Ca, Fn | ||
4SP + L.GG | Ss, Aa, Ca, Fn, L.GG | ||
5SP | Ss, Aa, Ca, Fn, Sm | ||
5SP + L.GG | Ss, Aa, Ca, Fn, Sm, L.GG |