High-throughput sequencing of 16S rRNA Gene Reveals Substantial Bacterial Diversity on the Municipal Dumpsite

BMC Microbiology

Table 4 Summary of good quality sequences and diversity indices of bacteria from different solid waste at species level

^bSamples	Valid reads	OTUs	ACE^a	Chao1^a	Shannon^a	InvSimpson^a
Dom	3,466,427	26,243	7338 ± 230	6788 ± 244	5 ± 1.0	26.9 ± 0.7
Biom	1,706,442	18,994	8218 ± 249	6756 ± 257	5.1 ± 0.01	54.2 ± 1.3
Riv	926,648	15,025	10,422 ± 283	8219 ± 287	5.6 ± 0.01	58.5 ± 1.2
FecD	2,369,595	16,697	6183 ± 214	5073 ± 226	4.5 ± 0.01	31.2 ± 0.9

OTUs Operational taxonomic units, ACE abundance based coverage estimator
^aCalculations were performed using Mothur package with an OTU definition at > 97 % sequence similarity
^bSamples: Dom—Domestic solid waste; Biom—Solid biomedical waste; Riv—River sludge; FecD—Faecal material of pigs scavenging on dumpsite

ISSN: 1471-2180