Site-specific bacterial chromosome engineering mediated by IntA integrase from Rhizobium etli

BMC Microbiology

Table 1 Oligonucleotides used in this work

Name	Sequence ^a	Genome location ^b	Source or reference
chr_left_in_hind	AAAAAAGCTTTCCCGGCTCCGACAG	1108084 Chr	This work
chr_right_in_eco	AAAAGAATTCCCGGTGTCTGCTTCCA	1108560 Chr	This work
chr_left_out	CGGAACACCGGATCTCA	1107995 Chr	This work
chr_right_out	CGTGCCCGCTTTTGTC	1108840 Chr	This work
Plac-out	CGCGGAACCCCTATTTG		10
CasNot-Ter-out	CCGTCTGTGATGGCTTCC		10
UpattA1	CTGGATCCCAAAGGTCGCTCCTGAAT	2252 p42a	8
LwattA1	CGAATTCATTTTCCCGAGAATAACATG	2687 p42a	8
Lw23bp	CGGATCCTCCGATAAGCATTACTTATCGGACTTAAGT	5765 p42d	8
Up23bp	CGGATCCTCCGATAAGCATTACTTATCGGACTTAAGT	5743 p42d	8
M13 reverse	CAGGAAACAGCTATGAC		ThermoFisher Scientific
LwattD1	CGAATTCCGGCGTTTGCATCTCGTTA	5875 p42d	8
ChrleftD	CTGGATCCTCCGATAAGTAA		This work
ChrightD	CGAATTCTGATTTGCGGGCG		This work
G18lw	CGGATCCTCCGATAAGCATTACTTATCGGACTTAAGT	attG18mob	This work
G18up	GCCTAGGAGGCTATTCGTAATGAATAGCCTGAATTCA	attG18mob	This work
Link-PstxNot	AGCGGCCGCTTGCA	NA

^aAll oligonucleotides are shown in the 5′ to 3′ direction. Built-in restriction sites, depicted in italics, are EcoRI (GAATTC) BamHI (GGATCC) HindIII (AAGCTT) NotI (GCGGCCGC)
^bThe location is indicated by the first 5′ nucleotide and the replicon where the sequence is located. Accession numbers are p42a (NC_007762), p42d (NC_004041), Chr (NC_007761) of R. etli. NA, not applicable (NA)

ISSN: 1471-2180