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Fig. 5 | BMC Microbiology

Fig. 5

From: Site-specific bacterial chromosome engineering mediated by IntA integrase from Rhizobium etli

Fig. 5

Flow cytometry analyses. For each sample, data from a minimum of 107 cells serially subcultured in the absence of antibiotics, for a period representing 21 generations were collected using ImageStream X imaging flow cytometer and analyzed using IDEAS 5.0 software. a cells with insertion of the LP sector on the chromosome display strong fluorescence. b cells with pK18 mob att Δplac inserted in the LP sector. c Mixture of cells corresponding to integration of pK18 mob att Δplac in the LP sector (107 cells) and the strain harboring the LP sector (103 cells)

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