Fig. 5
From: Alpha-1-antitrypsin interacts with gp41 to block HIV-1 entry into CD4+ T lymphocytes
AAT, C and ΔAAT had no obvious effect on CD4, CCR5 and CXCR4 expression on CD4+ T cells. CD4+ T cells were incubated in the presence or absence of AAT/C/ΔAAT and then collected to extract whole cell proteins. The expression of CD4, CCR5 and CXCR4 was detected by Western blot. β-actin was detected as the loading control (a). Meanwhile, the membrane level of CD4 (b), CCR5 (c) and CXCR4 (d) was also analyzed on these CD4+ T cells using flow cytometry. Ct: control group without reagent treatment