Fig. 1
From: Alpha-1-antitrypsin interacts with gp41 to block HIV-1 entry into CD4+ T lymphocytes
AAT inhibited HIV-1 replication. Activated primary CD4+ T cells were divided into two aliquots. One aliquot was treated with AAT (0.5, 1, or 5 g/L) for 1 h and then infected with HIV-1NL4.3 for 2 more hours without removing AAT. After infection, unbound viruses were removed by washing the T lymphocytes for three times and cells were then cultured with AAT as before infection. The other aliquot was directly infected with HIV-1NL4.3 for 2 h and unbound viruses were then removed by washing three times. Infected T cells were then treated with the presence or absence of AAT. HIV-1 production was detected by measuring HIV-1 p24 (in the supernatant) at different time point in each group