Table 1 Multiple sequence alignment of vertebrate TFPI-2 C-terminal region showing identical and similar amino acids

MAMMALS

P48307

Homo sapiens

Human

CAKALKKKKKMPKLRFASRIRKIRKKQF

14

H2QUX8

Pan troglodytes

Chimpanzee

CAKALKKKKKMPKLRFASRIRKIRKKQF

14

U3ENJ1

Callithrix jacchus

White-tufted-ear marmoset

CEKALKKQRKMPKIPFSNRNLKIWKKQF

9

F7CWN2

Macaca mulatta

Rhesus macaque

CAKALKRKKKIPKFRFASRIRKIRKKQF

14

XP_003896360.1

Papio anubis

Olive baboon

CAKALKRKKKIPKFRFASRIRKIRKKQF

14

H2PMX0

Pongo abelii

Sumatran orangutan

CAKALRKKKKMPKLRFASRIRKIRKKQF

14

G3QPC8

Gorilla gorilla gorilla

Western lowland gorilla

CAKALKRKKKMPKLRFASRIRKIRKKQF

14

XP_008067041.1

Tarsius syrichta

Philippine tarsier

CVKVLKKKTKVPKLLFANRRRKMQKKQF

12

H0XT57

Otolemur garnettii

Small-eared galago

CAKALKKEKNKTMPKLPFVNRILKVRK

9

O35536

Mus musculus

Mouse

CVKGWKKPKRWKIGDFLPRFWKHLS

7

XP_005363735.1

Microtus ochrogaster

Prairie Vole

CVKAPKKPKKRKTGSGFRIRTKPRRWTP

12

XP_004602345.1

Sorex araneus

European shrew

CANAMKKYKIKKLQRRHPSRRRSLKIKK

13

EDL84392.1

Rattus Norvegicus

Brown Rat

CVKALKKPKRRKIGDFLPRFWKLRS

9

XP_010601797.1

Fukomys damarensis

Damaraland Mole Rat

CEKAYKRGKRKTQKPPFSIKRPKSWRKR

12

H0VU83

Cavia porcellus

Guinea pig

CEKAYKRGKRKTQIPPFSIRRPKSWKKV

10

XP_007522577.1

Erinaceus europaeus

Western European Hedgehog

CAKASKKGKIRKMPRGILVNRRETYKKK

11

XP_011361893.1

Pteropus vampyrus

Large Flying Fox

CIKALKKKQNKKMPKILFVNRNLKMQKK

11

G1PX03

Myotis lucifugus

Little brown bat

CMKALRKKINKKMPRFFFPDRRRKLQKK

12

XP_006834353.1

Chrysochloris asiatica

Cape Golden Mole

CTLALKKENNKKMRKSFITNRRLKMRKR

11

XP_008569808.1

Galeopterus variegatus

Sunda flying lemur

CAKVKKGKNKKILKFPFVVRNLKFRKKK

13

XP_004475751.1

Dasypus novemcinctus

Nine-banded Armadillo

CENALKKEKKKKIPKGFFAIRRPKIQKK

10

W5NS10

Ovis aries

Sheep

CVKALKKEKNKKMPRLLFANRRLKIKKQ

11

M3X7K4

Felis silvestris catus

Cat

CVKALKKEKNKKMPKLFFANRRLKIWKR

11

G3THG9

Loxodonta africana

African elephant

CTKALKKKKKIQKPLFANRSPQIRK

10

F1SFC1

Sus scrofa

Pig

CVKALKKEKKMPRLLLASRRLKIKKKQF

11

E2RBF0

Canis familiaris

Dog

CVKALKKERNKKMTKLSLVNRRLKNWKK

11

G1MHA9

Ailuropoda melanoleuca

Giant panda

CVKGSRNEKNMQLLKTVFPRRRLKTYKN

8

Q7YRQ8

Bos taurus

Bovine

CVKALKKEKNKKMPRLLLANRRLKIKKK

12

XP_008684473.1

Ursus maritimus

Polar bear

CVKGSRKEKNMKLLKTVFPGRRLKTYKK

10

XP_008542988.1

Equus przewalskii

Przewalski’s horse

CAKGLKKKKNKKMKTLFLANRSLKLQKK

12

XP_007938042.1

Orycteropus afer afer

Aardvark

CVGFSLKKEKRKKIQKVLFANRRLKIRK

11

XP_007076762.1

Panthera tigris altaica

Amur Tiger

CVKALKKEKNKKMPKLFFANKRLKIWKR

11

XP_004431409.1

Ceratotherium simum simum

Southern White Rhinoceros

CTKALKKKKNRKMPKLFLANRSLKLRRK

13

XP_004386213.1

Trichechus manatus latirostris

Florida manatee

CAKALKKKKKKIRKFVLASRSLKLQKKQ

13

XP_006733001.1

Leptonychotes weddellii

Weddell seal

CIKAVRKQKDKKTPKIVFAHRRVKTWKK

11

XP_007450700.1

Lipotes vexillifer

Yangtze River dolphin

CVKALKKGKNKKMPKLLFASRRLKIKKK

13

XP_004265641.1

Orcinus orca

Killer Whale

CVKALKKGKNKKMPKLLFASRRLKIKKK

13

BIRDS

XP_010710984

Meleagris gallopavo

Wild Turkey

CKKGSQKPTISKPRNLLRRKMMRKLIKK

12

XP_009328855.1

Pygoscelis adeliae

Adélie penguin

CRKGTQKPRINKPMNVFRRKVMRKLIKK

12

XP_010560815

Haliaeetus leucocephalus

Bald eagle

CRKAGTQKPRINKPTNVFRRKMMRKLIK

11

XP_005228880.1

Falco peregrinus

Peregrine falcon

CRKGTQKPRINKPANVFRRKMMRKLTKK

12

XP_009934468.1

Opisthocomus hoazin

Hoatzin

CRKGTEKPRINKPTNVFRRKMMRKLVKK

11

XP_009893654.1

Charadrius vociferus

Killdeer

CRKAGAQKPRIDKPTNVFRRKMMRKLIK

10

XP_009639692.1

Egretta garzetta

Little egret

CRKGTEKPGINKPMNVFRRKMMRKLTKK

10

XP_009672625.1

Struthio camelus australis

Ostrich

CRKGSEKPRINKPANLLRRKMMRKLIKK

11

XP_009464906.1

Nipponia nippon

Crested Ibis

CRKGTEKPRINKPTNVFRRKMMRKLIKK

11

XP_009084577.1

Serinus canaria

Atlantic canary

CRKAGNQKPSINKPTNLSRRKIVRKLKK

11

XP_008919443.1

Manacus vitellinus

Golden-collared manakin

CRKGTQKPRINKPMSVSRRKIMRKLIKK

12

XP_008492487.1

Calypte anna

Anna’s hummingbird

CRKAGTQKPKINKPVNGVRRKMMIKLIK

11

XP_005518860.1

Pseudopodoces humilis

Ground tit

CGKGNQKLRINKPTNVSRRKIVRKLIEK

9

XP_005152979.1

Melopsittacus undulatus

Budgerigar

CRKGTQKPGINKPMNVFRRKMMRKLVKK

11

XP_005041216.1

Ficedula albicollis

The collared flycatcher

CRNAGNQKLRINKPTNVSRRKLVRKLIK

10

XP_005010988.1

Anas platyrhynchos

Mallard

CGKAGSQKPAMKKSTDVFRRKMMRKLIK

9

XP_418662.2

Gallus gallus

Chicken

CRKGSQKPTINKSRSLLRRKMMRKLIKK

12

XP_005418689.1

Geospiza fortis

Medium ground finch

CRNGNQKPGINKPTNLSRRKIVRKLKKK

11

XP_005480303.1

Zonotrichia albicollis

White-throated Sparrow

CRNAGNQKPAINKPANLSRRKIVRKLKK

10

REPTILES

XP_005308608.1

Chrysemys picta bellii

Western painted turtle

CKKAGSKKSSFKKSRNKLPKTMRKLPEK

11

XP_006258519.1

Alligator Mississippiensis

American Alligator

CRKAGNKKPRIKPKSKVMRKMMRKLQKN

13

AMPHIBIANS

Q5FVY6

Xenopus tropicalis

Western clawed frog

CKKGSKRPRNRNRIRVPRIQS

9

FISHES

XP_010889816.1

Esox lucius

The northern pike

CAKAGKLWKTKNPVTKTAMMKIPKKRSR

10

XP_010739844.1

Larimichthys crocea

Croceine croaker

CAKGGKKYKRQGKGRRMRRYRNNNITFL

11

XP_008419957.1

Poecilia reticulata

Guppy

CVRGPKRHSSHGKLRRLRQNRNNHLPLH

8

XP_008329541.1

Cynoglossus semilaevis

Cynoglossus

CVKGEKKHTGQGMIRRLRRNKNNSIFVV

7

XP_008287494.1

Stegastes partitus

Bicolor damselfish

CVKGAKKQTGQGKGRRMRRNRHNHITFL

9

XP_007259866.1

Mexican tetra

Astyanax mexicanus

CARKSKPGKMRRKLIPRKPERRI

10

C3KHI5

Anoplopoma fimbria

Sablefish

CVTGRKHTIQSKGRRLRRNRINRITFRQ

10

Q1WCN6

Danio rerio

Zebrafish

CGSKRWSPTKKSVRVSKQYLRRVKPQPS

9

I3JXJ8

Oreochromis niloticus

Nile tilapia

CAKGWKKHPGRGKILRSRKNRKLVTFLQ

10

XP_003969359.1

Takifugu rubripes

Japanese Puffer

CVRGRKIRTREGKTNPLRRNRNNRITFM

9

XP_004073873.1

Oryzias latipes

Japanese Rice Fish

CLKGAKKHTGLKGRDRLMKRNKKTRFMA

10

XP_010772945.1

Notothenia coriiceps

Black Rock Cod

CARRGKKLTSQVKGRRKRRNRNNRITFL

12

XP_007903512.1

Callorhinchus milii

Australian Ghostshark

CQKRNKKKTSQKYPTASITRKVVKKNSR

11