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Table 2 Primers used in this study

From: Shigella flexneri serotype 1c derived from serotype 1a by acquisition of gtrIC gene cluster via a bacteriophage

Primer name

Sequence

Description

GtrIc-F(BamHI)a

TTAGGATCCAGGGATTCAACTGATTGGC

Binds upstream of gtrIC (in gtrB IC ).

GtrIcR2-BamHI

TGAGGATCCGACAGGATCAATCACCGC

Binds downstream of gtrIC stop codon.

DG_GtrA(Ic)F(SacI) b

GTCGAGCTCTTGATGCTAAACTCTCACTTT

Binds to sequence 24–44 bp upstream of gtrA IC start codon

  1. aPrimer pair of GtrIc-F(BamHI) and GtrIc-R2(BamHI), was optimal for the production of a single band which corresponded to the gtrIC gene
  2. bPrimer pair of DG_GtrA(Ic)F(SacI) and GtrIc-R2(BamHI) was the best choice for the gtrIC gene cluster
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BMC Microbiology

ISSN: 1471-2180

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