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Fig. 4 | BMC Microbiology

Fig. 4

From: Shigella flexneri serotype 1c derived from serotype 1a by acquisition of gtrIC gene cluster via a bacteriophage

Fig. 4

Southern Blot of Eco32I digested chromosomal DNA with a gtrIC probe. i. Agarose gel of digested genomic DNA. ii. Southern blot analysis of digested genomic DNA. a Egyptian serotype 1c strains. Lane 1. Marker SPP-I/EcoRI; 2. SFL1613 (control strain); 3. SFL1683; 4. SFL1684; 5. SFL1686; 6.SFL1687; 7. SFL1688; 8.SFL1689; 9. SFL1690; 10.SFL1691; 11.SFL1692; 12. SFL1685. b Bangladeshi serotype 1c strains. Lane 1. Marker SPP-I/EcoRI; 2. SFL1613 (control strain); 3. SFL1500; 4. SFL1502; 5. SFL1503. c Vietnamese (Son Tay Province) serotype 1c strains. Lane 1. SPP-I/EcoRI; 2. SFL1613 (control strain); 3. SFL1564; 4. SFL1568; 5. SFL1569; 6.SFL1571; 7. SFL1575; 8.SFL1576; 9. SFL1577; 10.SFL1578; 11.SFL1579; 12. SFL1570. d Vietnamese Serotype 1c strains - Son Tay Province. (Isenbarger et al. 2001). Lane 1. SFL1580; 2. SFL1581; 3. SFL1582; 4. SFL1583; 5. SFL1584; 6.SFL1585; 7. SFL1586; 8.SFL1587; 9. SFL1588; 10.SFL1589; 11.SFL1590; 12. SFL1594.; 13.SFL1596; 14. SFL1597. 15.SFL1598; 16. SFL1600; 17.SFL1602; 18. SFL1603;19. SFL1613 (positive control strain) 20. Marker SPP-I/EcoRI. e Nha Trang, Vietnam (5 from Isenbarger et al. 2001, 13 from Prof. Cam PD). Lane 1. Marker SPP-I/EcoRI; 2. SFL1604; 3. SFL1605; 4. SFL1606; 5. SFL1607; 6.SFL1610; 7. SFL1556; 8.SFL1557; 9. SFL1558; 10.SFL1561; 11.SFL1562; 12. SFL1565.; 13.SFL1566; 14. SFL1567. 15.SFL1572; 16. SFL1573; 17.SFL1599; 18. SFL1712;19. SFL1613 (positive control strain) 20. Marker SPP-I/XbaI

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