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Fig. 3 | BMC Microbiology

Fig. 3

From: Auxotrophic Actinobacillus pleurpneumoniae grows in multispecies biofilms without the need for nicotinamide-adenine dinucleotide (NAD) supplementation

Fig. 3

CLSM of multi-species biofilms of A. pleuropneumoniae with other swine pathogens without NAD supplementation. A. pleuropneumoniae, S. suis, B. bronchiseptica, P. multocida, S. aureus and E. coli grown as single, dual or triple-species biofilms in BHI media without NAD stained with FM 1-43, SYTO 9, propidium iodide, wheat-germ agglutinin (WGA)-Oregon green, BOBO-3, and SYPRO Ruby (all from Invitrogen, Eugene, OR). PGA: poly-N-acetylglucosamine; eDNA: extracellular DNA; App: A. pleuropneumoniae; Ss: S. suis; Bb: B. bronchiseptica; Pm: P. multocida; Sa: S. aureus; Ec: E. coli. Scale bar 30 μm

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BMC Microbiology

ISSN: 1471-2180

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