5-azacytidine induces transcriptome changes in Escherichia coli via DNA methylation-dependent and DNA methylation-independent mechanisms

Table 3 RT-qPCR analysis of gene expression changes in 5-azacytidine treated and dcm knockout Escherichia coli

Gene	Phase	fc (log₂) + aza/-aza (array)	fc (log₂) + aza/-aza (RT-qPCR)	fc (log₂) Δdcm/wild-type (RT-qPCR)
recA	log	2.96	3.65^a	0.68
rsmI	log	1.82	3.54^b	2.86^a
atpH	early stationary	1.66		0.64
cspB	early stationary	3.40		1.46^a
fimA	early stationary	2.11		1.46^a
osmE	early stationary	−1.46		−1.79^a
recA	early stationary	2.53	2.98^b	0.98
rpoS	early stationary	0.69	0.035	0.17
osmE	stationary			−3.0^a
rpoS	stationary			−0.15

ISSN: 1471-2180