Fig. 2

The effect of 5-azaC on Escherichia coli growth in liquid media. E. coli wild-type strain BW25113 was grown in LB at 37 °C in the absence of 5-azaC (black squares), the presence of 0.5 μg/mL 5-azaC (red circles), 5 μg/mL 5-azaC (green triangles) and 50 μg/mL 5-azaC (blue diamonds) for eight hours. At every hour, each culture was analyzed by spectrophotometry at 600 nm. The data are from three biological replicates (n = 3). Error bars representing standard deviation are not shown to maintain clarity of the four lines. Brackets indicate time points of RNA isolation for microarray analysis, and data analyzed by ANOVA. The asterisk indicates a p-value of <0.05