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Table 4 Primers for amplification of DNA for sequencing on the Ion PGM platform

From: 16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform

 

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Primer

Ref

V4-V5

    

[23]

 Forward barcoded primers

     

  Mock DNA

CCATCTCATCCCTGCGTGTCTCCGACTCAG

TCCCTTGTCTCC

GT

GTGCCAGCMGCCGCGGTAA

 

  PBS Qiagen

CCATCTCATCCCTGCGTGTCTCCGACTCAG

ACGAGACTGATT

GT

GTGCCAGCMGCCGCGGTAA

 

  PBS RBB

CCATCTCATCCCTGCGTGTCTCCGACTCAG

GCTGTACGGATT

GT

GTGCCAGCMGCCGCGGTAA

 

  Glycerol Qiagen

CCATCTCATCCCTGCGTGTCTCCGACTCAG

ATCACCAGGTGT

GT

GTGCCAGCMGCCGCGGTAA

 

  Glycerol RBB

CCATCTCATCCCTGCGTGTCTCCGACTCAG

TGGTCAACGATA

GT

GTGCCAGCMGCCGCGGTAA

 

 Reverse primer

CCTCTCTATGGGCAGTCGGTGAT

 

CC

CCGTCAATTYYTTTRAGTTT

 

V1-V2 set 1

    

[4]

 Forward barcoded primers

     

  Mock DNA

CCATCTCATCCCTGCGTGTCTCCGACTCAG

TGCATACACTGG

GT

AGAGTTTGATCCTGGCTCAG

 

  PBS Qiagen

CCATCTCATCCCTGCGTGTCTCCGACTCAG

AGTCGAACGAGG

GT

AGAGTTTGATCCTGGCTCAG

 

  PBS RBB

CCATCTCATCCCTGCGTGTCTCCGACTCAG

ACCAGTGACTCA

GT

AGAGTTTGATCCTGGCTCAG

 

  Glycerol Qiagen

CCATCTCATCCCTGCGTGTCTCCGACTCAG

GAATACCAAGTC

GT

AGAGTTTGATCCTGGCTCAG

 

  Glycerol RBB

CCATCTCATCCCTGCGTGTCTCCGACTCAG

GTAGATCGTGTA

GT

AGAGTTTGATCCTGGCTCAG

 

 Reverse primer

CCTCTCTATGGGCAGTCGGTGAT

 

CC

TGCTGCCTCCCGTAGGAGT

 

V1-V2 set 2

    

[17]

 Forward barcoded primers

     

  Mock DNA

CCATCTCATCCCTGCGTGTCTCCGACTCAG

GCGATATATCGC

GT

AGMGTTYGATYMTGGCTCAG

 

  PBS Qiagen

CCATCTCATCCCTGCGTGTCTCCGACTCAG

CGAGCAATCCTA

GT

AGMGTTYGATYMTGGCTCAG

 

  PBS RBB

CCATCTCATCCCTGCGTGTCTCCGACTCAG

AGTCGTGCACAT

GT

AGMGTTYGATYMTGGCTCAG

 

  Glycerol Qiagen

CCATCTCATCCCTGCGTGTCTCCGACTCAG

GTATCTGCGCGT

GT

AGMGTTYGATYMTGGCTCAG

 

  Glycerol RBB

CCATCTCATCCCTGCGTGTCTCCGACTCAG

CGAGGGAAAGTC

GT

AGMGTTYGATYMTGGCTCAG

 

 Reverse primer

CCTCTCTATGGGCAGTCGGTGAT

 

CC

TGCTGCCTCCCGTAGGAGT

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