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Table 4 Sizes (bp) of restriction fragments obtained by cleavage of 16S rDNA amplicons of Lactobacillus strains isolated from poultry. The values in brackets refer only some strains

From: 16S-ARDRA and MALDI-TOF mass spectrometry as tools for identification of Lactobacillus bacteria isolated from poultry

Group

No. of isolates

Identification

Size (bp) of restriction fragments found in wild strains

   

MseI

HinfI

MboI

AluI

L. salivarius

17

L. salivarius

350, 256, 145, 130

900, 385, 190

1070, 190, 118

840, 265, 205

2

L. saerimneri

400, 350, 256, 237, 202, 130

900, 385, 190

1070, 324

570, 270, 250, 210, 97

4

L.agilis

350, 256, 145, 130

900, 385, 190

1070, 312

720, (363), 205, 180, 80

L. plantarumm

3

L. plantarum

(520), 470, 295, 256, 145, 130, 110, 90

910, 385, 190

1300, 175

570, 265, 205, 97

L. alimentarius

2

L. farciminis

620, 295, 256, 202, 130, 90

540, 390, 300, 200

(850), (470), (395), 235, 200, 175,

570, 265, 205, (130), (111), (80)

L. reuteri

11

L. ingluviei

(650), 450, 256, 237, 202, 130, 90

900, 385, 200

850, 324, 180, 165

410, 265, 210, 205, 180, 100

2

L. mucosae

450, 256, 237, 202, 130, 90

(910), 900, 200, 170

850, 324, 180, 165

(570), 410, 265, 205, 180, 100

8

L. reuteri

650, (450), 256, 145, 130, 90

(1170), (910), 900, 385, 200

850, 324, 180, 165

(570), 410, 265, 205, 180, 100

4

L. oris

650, (550), (430), 256, 202, 130, 90

910, 290, 200, 110

850, (480), 324, 180, 165

570, 270, 180, 100

L. delbruckii

12

L. johnsonii

940, 256, 145, 130, 90

910, 385, 85

920, 324, 180

410, 250, 205, 180

8

L. crispatus

450, 256, 237, 130, 90

900, 385, 120, 85

920, 190, 175, 118

410, 210, 205, 180

2

L. amylovorus

650, 450, 256, 130, 90

900, 385, 120, 85

920, 190, 175, 118

410, 210, 205, 180

1

L. kitasatonis

450, 256, 237, 145, 130, 90

900, 385, 120, 85

920, 190, 175, 118

410, 210, 205, 180

L. casei

3

L. paracasei

470, 256, 202, 130, 110, 90

910, 290, 200

700, 330, 175, 130, 118

570, 265, 230, 205

1

L. rhamnosus

470, 256, 202, 130, 110, 90

910, 385, 200

700, 330, 312, 175

570, 265, 230, 205

Total: 80

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