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Table 2 Primers used for detecting Salmonella by LAMP-BART and conventional LAMP, in comparison with the primer set published previously

From: Rapid detection of Salmonella in food and feed by coupling loop-mediated isothermal amplification with bioluminescent assay in real-time

Primer name

Sequence (5′-3′) a

Position b

Reference

Primer set optimized for LAMP-BART and also used in conventional LAMP

This study

 Sal4-F3

GAACGTGTCGCGGAAGTC

484-501

 

 Sal4-B3

CGGCAATAGCGTCACCTT

665-682

 Sal4-FIP

GCGCGGCATCCGCATCAATA-

TCTGGATGGTATGCCCGG

573-592 (F1c)

516-533 (F2)

 Sal4-BIP

GCGAACGGCGAAGCGTACTG-

TCGCACCGTCAAAGGAAC

593-612 (B1c)

635-652 (B2)

 Sal4-Loop-F

TCAAATCGGCATCAATACTCATCTG

538-562

 Sal4-Loop-B

AAAGGGAAAGCCAGCTTTACG

614-634

Primer set published in a previous study

[14]

 F3

CGGCCCGATTTTCTCTGG

503-520

 

 B3

CGGCAATAGCGTCACCTT

665-682

 

 FIP

GCGCGGCATCCGCATCAATA-

TGCCCGGTAAACAGATGAGT

573-592 (F1c)

527-546 (F2)

 

 BIP

GCGAACGGCGAAGCGTACTG-

TCGCACCGTCAAAGGAAC

593-612 (B1c)

635-652 (B2)

 

 Loop-F

GGCCTTCAAATCGGCATCAAT

547-567

 

 Loop-B

GAAAGGGAAAGCCAGCTTTACG

613-634

 
  1. aUnderlined sequences were either F2 or B2 as indicated. bThe positions are numbered based on the coding sequence of the Salmonella invA gene [GenBank: M90846]